Identification of bioactive peptides released from in vitro gastrointestinal digestion of yam proteins (Dioscorea cayennensis)

Edilza Silva do Nascimento; Katya Anaya; Julia Mariano Caju de Oliveira; José Thalles Jocelino Gomes de Lacerda; Michael Edward Miller; Meriellen Dias; Maria Anita Mendes; Juliana de Azevedo Lima Pallone; Clarice Weis Arns; Maria Aparecida Juliano; Tatiane Santi Gadelha; Maria Teresa Bertoldo Pacheco; Carlos Alberto de Almeida Gadelha

doi:10.1016/j.foodres.2021.110286

Identification of bioactive peptides released from in vitro gastrointestinal digestion of yam proteins (Dioscorea cayennensis)

Food Res Int. 2021 May:143:110286. doi: 10.1016/j.foodres.2021.110286. Epub 2021 Mar 9.

Authors

Affiliations

¹ Department of Food Engineering, Post-Graduate Program in Food Science and Technology, Technology Center, Federal University of Paraiba, João Pessoa, PB, Brazil; Department of Molecular Biology, Laboratory of Structural Proteomics, Federal University of Paraíba, João Pessoa, PB, Brazil. Electronic address: edilsa_nat@hotmail.com.
² Faculty of Health Sciences of Trari, Federal University of Rio Grande do Norte, Santa Cruz, RN, Brazil. Electronic address: katya_jacinto@yahoo.com.br.
³ Department of Molecular Biology, Laboratory of Structural Proteomics, Federal University of Paraíba, João Pessoa, PB, Brazil. Electronic address: juliacaju0@gmail.com.
⁴ Department of Biophysics, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: thalles_lacerda2@hotmail.com.
⁵ Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas, Campinas, SP, Brazil. Electronic address: mike@millerpost.com.
⁶ Department of Chemical Engineering, University of São Paulo, São Paulo, SP, Brazil. Electronic address: meriellend@gmail.com.
⁷ Department of Chemical Engineering, University of São Paulo, São Paulo, SP, Brazil. Electronic address: mariaanita.mendes@gmail.com.
⁸ Department of Food Science, School of Food Engineering, State University of Campinas, Campinas, SP, Brazil. Electronic address: jpallone@unicamp.br.
⁹ Department of Genetics, Evolution and Bioagents, Institute of Biology, State University of Campinas, Campinas, SP, Brazil. Electronic address: arns@unicamp.br.
¹⁰ Department of Biophysics, Federal University of São Paulo, São Paulo, SP, Brazil. Electronic address: ma.juliano@unifesp.br.
¹¹ Department of Molecular Biology, Laboratory of Genetic Biochemistry and Radiology, Federal University of Paraíba, João Pessoa, PB, Brazil. Electronic address: santi.tatiane@gmail.com.
¹² Institute of Food Technology, Center of Food Science and Quality, Campinas, SP, Brazil. Electronic address: mtb@ital.sp.gov.br.
¹³ Department of Food Engineering, Post-Graduate Program in Food Science and Technology, Technology Center, Federal University of Paraiba, João Pessoa, PB, Brazil; Department of Molecular Biology, Laboratory of Structural Proteomics, Federal University of Paraíba, João Pessoa, PB, Brazil. Electronic address: calbgadelha@gmail.com.

PMID: 33992386
DOI: 10.1016/j.foodres.2021.110286

Abstract

Bioactive peptides have been broadly studied for their contribution to human health. This study aimed to identify bioactive peptides generated by in vitro gastrointestinal digestion of yam proteins. Yam protein concentrate (YPC) was submitted to simulated digestion. Gastric phase hydrolysate (GPH) and total gastrointestinal phase hydrolysate (GIPH) had their peptides identified by nanoLC-ESI-MS/MS. Peptide sequences were subjected to a database-driven (BIOPEP) bioactivity search. In vitro tests included: Antioxidant activity, DNA damage protection, ACE-inhibitory activity and antibacterial activity against the bacteria Escherichia coli, Salmonella sp. and Lysteria monocytogenes. Simulated digestion generated small peptides (mostly MW < 3500 Da), several of them with potential bioactive sequences predicted in silico. In both GPH and GIPH biological activities were detected, although GIPH displayed stronger DNA damage protection and antibacterial activity against Escherichia coli. The digestion of yam proteins releases promising biologically active peptides which can contribute to the prevention of bacterial infection and chronic degenerative diseases, with beneficial effects to human health.

Keywords: Angiotensin I-Converting Enzyme (ACE) inhibitory activity; Antibacterial activity; Antioxidant activity; BIOPEP; Bioactive potential; NanoLC-ESI-MS/MS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Digestion
Dioscorea*
Humans
Peptides
Tandem Mass Spectrometry

Substances

Peptides