TRPC1-mediated Ca2+ signaling enhances intestinal epithelial restitution by increasing α4 association with PP2Ac after wounding

Navneeta Rathor; Hee Kyoung Chung; Jia-Le Song; Shelley R Wang; Jian-Ying Wang; Jaladanki N Rao

doi:10.14814/phy2.14864

TRPC1-mediated Ca²⁺ signaling enhances intestinal epithelial restitution by increasing α4 association with PP2Ac after wounding

Physiol Rep. 2021 May;9(9):e14864. doi: 10.14814/phy2.14864.

Authors

Navneeta Rathor^{1

2}, Hee Kyoung Chung^{1

2}, Jia-Le Song¹, Shelley R Wang², Jian-Ying Wang^{1

2

3}, Jaladanki N Rao^{1

2}

Affiliations

¹ Cell Biology Group, Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.
² Baltimore Veterans Affairs Medical Center, Baltimore, MD, USA.
³ Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, USA.

Abstract

Gut epithelial restitution after superficial wounding is an important repair modality regulated by numerous factors including Ca²⁺ signaling and cellular polyamines. Transient receptor potential canonical-1 (TRPC1) functions as a store-operated Ca²⁺ channel in intestinal epithelial cells (IECs) and its activation increases epithelial restitution by inducing Ca²⁺ influx after acute injury. α4 is a multiple functional protein and implicated in many aspects of cell functions by modulating protein phosphatase 2A (PP2A) stability and activity. Here we show that the clonal populations of IECs stably expressing TRPC1 (IEC-TRPC1) exhibited increased levels of α4 and PP2A catalytic subunit (PP2Ac) and that TRPC1 promoted intestinal epithelial restitution by increasing α4/PP2Ac association. The levels of α4 and PP2Ac proteins increased significantly in stable IEC-TRPC1 cells and this induction in α4/PP2Ac complexes was accompanied by an increase in IEC migration after wounding. α4 silencing by transfection with siRNA targeting α4 (siα4) or PP2Ac silencing destabilized α4/PP2Ac complexes in stable IEC-TRPC1 cells and repressed cell migration over the wounded area. Increasing the levels of cellular polyamines by stable transfection with the Odc gene stimulated α4 and PP2Ac expression and enhanced their association, thus also promoting epithelial restitution after wounding. In contrast, depletion of cellular polyamines by treatment with α-difluoromethylornithine reduced α4/PP2Ac complexes and repressed cell migration. Ectopic overexpression of α4 partially rescued rapid epithelial repair in polyamine-deficient cells. These results indicate that activation of TRPC1-mediated Ca²⁺ signaling enhances cell migration primarily by increasing α4/PP2Ac associations after wounding and this pathway is tightly regulated by cellular polyamines.

Keywords: IEC-6 cells; TRPC1; cell migration; epithelial restitution; mucosal injury; ornithine decarboxylase; polyamines.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Adaptor Proteins, Signal Transducing / metabolism*
Animals
Calcium Signaling*
Cell Line
Cell Movement
Enterocytes / metabolism*
Molecular Chaperones / metabolism*
Polyamines / metabolism
Protein Phosphatase 2 / metabolism*
Rats
TRPC Cation Channels / metabolism*
Wound Healing*

Substances

Adaptor Proteins, Signal Transducing
Igbp1 protein, rat
Molecular Chaperones
Polyamines
TRPC Cation Channels
Trpc2 protein, rat
Protein Phosphatase 2

Abstract

Publication types

MeSH terms

Substances

Grants and funding