Developmental validation of the Microreader™ Y Prime Plus ID System: An advanced Y-STR 38-plex system for forensic applications

Yi Zhao; Xiaojuan Yao; Yifan Li; Chengjian Chen; Dan Guo; Zhangping Jiao; Zhipan Yang; Zailiang Yu; Wanshan Ma; Qingxia Zhang

doi:10.1016/j.scijus.2021.01.003

Developmental validation of the Microreader™ Y Prime Plus ID System: An advanced Y-STR 38-plex system for forensic applications

Sci Justice. 2021 May;61(3):260-270. doi: 10.1016/j.scijus.2021.01.003. Epub 2021 Jan 30.

Authors

Yi Zhao¹, Xiaojuan Yao², Yifan Li³, Chengjian Chen¹, Dan Guo³, Zhangping Jiao¹, Zhipan Yang³, Zailiang Yu⁴, Wanshan Ma⁵, Qingxia Zhang⁶

Affiliations

¹ Forensic Science Service of the Beijing Public Security Bureau, 100192 Beijing, China.
² Forensic Science Service of the Xinzhou Public Security Bureau, 034000 Shanxi, China.
³ Beijing Microread Genetics, 100045 Beijing, China.
⁴ Beijing Microread Genetics, 100045 Beijing, China. Electronic address: 532598384@qq.com.
⁵ Suzhou Microread Genetics, 215163 Suzhou, China. Electronic address: 13691442641@139.com.
⁶ Forensic Science Service of the Beijing Public Security Bureau, 100192 Beijing, China. Electronic address: qingxiazhang@sina.com.

PMID: 33985674
DOI: 10.1016/j.scijus.2021.01.003

Abstract

Y-STR is widely used in sexual assaults and familial searches of suspects. Here, we reported a novel 38-plex STR genotyping system designed for forensic applications. Microreader™ Y Prime Plus ID System (YPP) amplifies 38 loci in one reaction, including 29 loci from commonly used Yfiler® Plus PCR Amplification Kit & PowerPlex® Y23 System (DYS393, DYS570, DYS19, DYS392, DYS549, Y GATA H4, DYS460, DYS458, DYS481, DYS635, DYS448, DYS533, DYS449, DYS456, DYS389I, DYS390, DYS389Ⅱ, DYS438, DYS391, DYS439, DYS437, DYS385a/b, DYS643, DYS518, DYS576, DYF387S1a/b, and DYS627), 6 commonly used loci for the Y-STR database (DYS444, DYS447, DYS596, DYF404a/b, DYS527a/b, DYS557) and one Y-indel specific for the Chinese population. YPP is designed for different types of samples, such as blood card and swabs. In this work, YPP was validated following SWGDAM guidelines (2016) and guidelines from Ministry of Public Security of the People's Republic of China, including PCR-based, sensitivity, accuracy and precision, mixture, stability and inhibitor, and species specificity. The results indicate that the Microreader™ Y Prime Plus ID System is a powerful identification kit designed for forensic databases.

Keywords: Developmental validation; Microreader™ Y Prime Plus; Y-STR.

MeSH terms

Chromosomes, Human, Y*
DNA Fingerprinting* / methods
Genetics, Population
Humans
Microsatellite Repeats
Polymerase Chain Reaction
Species Specificity