Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs

Shiqi Li; Xiaoying Zhong; Yanan Xu; Yunquan Zheng; Xianai Shi; Feng Li; Shaobin Guo; Jianmin Yang

doi:10.1016/j.foodchem.2021.130019

Smartphone-based reading system integrated with phycocyanin-enhanced latex nanospheres immunoassay for on-site determination of aflatoxin B1 in foodstuffs

Food Chem. 2021 Oct 30:360:130019. doi: 10.1016/j.foodchem.2021.130019. Epub 2021 May 6.

Authors

Shiqi Li¹, Xiaoying Zhong¹, Yanan Xu¹, Yunquan Zheng², Xianai Shi³, Feng Li³, Shaobin Guo³, Jianmin Yang⁴

Affiliations

¹ College of Chemistry, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China.
² College of Chemistry, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China; Fujian Key Laboratory of Medical Instrument and Pharmaceutical Technology, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China. Electronic address: yunquanzheng@fzu.edu.cn.
³ College of Biological Science and Engineering, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China; Fujian Key Laboratory of Medical Instrument and Pharmaceutical Technology, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China.
⁴ College of Biological Science and Engineering, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China; Fujian Key Laboratory of Medical Instrument and Pharmaceutical Technology, Fuzhou University, No. 2 Xueyuan Road, Fuzhou 350108, China. Electronic address: jmyang@fzu.edu.cn.

PMID: 33975069
DOI: 10.1016/j.foodchem.2021.130019

Abstract

Traditional methods for aflatoxin B1 (AFB1) detection are complex, time-consuming, labor-intensive, and high cost. Moreover, they require sophisticated large-scale instrumentation, which limits their on-site rapid detection. Herein, phycocyanin fluorescent nanospheres based on fluorescence immunochromatographic assay were developed for quantitative detection of AFB1 at parts-per-billion (ppb) levels in foodstuffs. Phycocyanin and anti-AFB1 monoclonal antibodies were coupled on the surface of latex nanospheres to amplify the fluorescence signal and improve the sensitivity. The fluorescence intensity was measured by a self-developed smartphone-based reading system. Under the optimal conditions, this approach achieved quantitative point-of-care detection of AFB1 within 25 min. The calibration curve for AFB1 was linear in the range of 0.2-48 ppb, and the limit of detection was 0.16 ppb. The practical applicability of the proposed approach was demonstrated by the determination of AFB1 in naturally contaminated samples, and the results were consistent with HPLC detection.

Keywords: Aflatoxin B1; Immunoassay; On-site determination; Phycocyanin; Smartphone.

MeSH terms

Aflatoxin B1 / analysis*
Antibodies, Monoclonal / immunology
Food Contamination / analysis*
Immunoassay / methods
Latex
Limit of Detection
Nanospheres*
Phycocyanin / chemistry*
Smartphone*

Substances

Antibodies, Monoclonal
Latex
Phycocyanin
Aflatoxin B1