Within- and Trans-Generational Life History Responses to Diurnal Temperature Amplitudes of the Pupal Stage in the Diamondback Moth

Abstract

Diurnal temperature fluctuations in nature can have a significant effect on many ectodermic traits. However, studies on the effects of diurnal temperature fluctuations on organisms, especially the effects on specific life stages, are still limited. We examined the immediate effects of the same average temperature (25°C) and different temperature amplitudes (±4, ±6, ±8, ±10, ±12°C) on the development and survival of Plutella xylostella (Lepidoptera: Plutellidae). We also assessed carry-over effects on adult longevity, reproduction, development, and survival of offspring across generations. The effect of moderate temperature amplitudes was similar to that of constant temperature. Wide temperature amplitudes inhibited the development of pupae, reduced total reproduction, lowered intrinsic rates of population growth, and slowed the development and survival of eggs on the first day, but the proportion of females ovipositing on the first three days increased. Insects coped with the adverse effects of wide temperature amplitudes by laying eggs as soon as possible. Our results confirmed that a logistic model based on daily average temperature cannot predict development rates under wide temperature amplitudes. These findings highlight the effect of environmental temperature fluctuations at the pupal stage on the development and oviposition patterns of P. xylostella and should be fully considered when predicting field occurrence.

Keywords: Plutella xylostella; carry-over effect; pupal stage; thermal amplitude; transgenerational effect.