Orally Administered DHA-Enriched Phospholipids and DHA-Enriched Triglyceride Relieve Oxidative Stress, Improve Intestinal Barrier, Modulate Inflammatory Cytokine and Gut Microbiota, and Meliorate Inflammatory Responses in the Brain in Dextran Sodium Sulfate Induced Colitis in Mice

Hongxia Che; Hongyan Li; Lin Song; Xiufang Dong; Xihong Yang; Tiantian Zhang; Yuming Wang; Wancui Xie

doi:10.1002/mnfr.202000986

Orally Administered DHA-Enriched Phospholipids and DHA-Enriched Triglyceride Relieve Oxidative Stress, Improve Intestinal Barrier, Modulate Inflammatory Cytokine and Gut Microbiota, and Meliorate Inflammatory Responses in the Brain in Dextran Sodium Sulfate Induced Colitis in Mice

Mol Nutr Food Res. 2021 Aug;65(15):e2000986. doi: 10.1002/mnfr.202000986. Epub 2021 Jun 18.

Authors

Hongxia Che^{1

2}, Hongyan Li^{1

2}, Lin Song^{1

2}, Xiufang Dong^{1

2}, Xihong Yang^{1

2}, Tiantian Zhang³, Yuming Wang³, Wancui Xie^{1

2}

Affiliations

¹ College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, Shandong, 266042, China.
² Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao, Shandong, 266042, China.
³ College of Food Science and Engineering, Ocean University of China, No.5 Yushan Road, Qingdao, 266003, China.

PMID: 33974360
DOI: 10.1002/mnfr.202000986

Abstract

Scope: Studies based on DHA/EPA supplementation in animal models of inflammatory bowel disease (IBD) reveal controversial results. It is speculated that different forms of DHA may explain the controversial results. Therefore, the effects of DHA-enriched phospholipids (DHA-PL) and DHA-enriched triglyceride (DHA-TG) on IBD are compared.

Methods and results: Male C57BL6/J mice are given DHA-PL and DHA-TG for 14 consecutive days, and receive ad libitum a 3.0% dextran sodium sulfate solution on the eighth day to establish IBD model. The results show that both DHA-PL and DHA-TG can reverse the colitis pathological process by decreasing the disease activity indexes (DAI), raising the colon length, suppressing the intestinal permeability, suppressing the oxidative stress, down-regulating pro-inflammatory factors, up-regulating anti-inflammatory factor in colon tissues. DHA-PL and DHA-TG also regulate the composition of gut microbiota via decreasing of the abundance Bacteroidetes and Firmicutes, and DHA-TG increases the abundance of Odoribacter. Importantly, DHA-PL and DHA-TG obviously attenuate the activation of microglia.

Conclusions: DHA-PL shows outstanding advantages in regulating oxidative stress, inflammatory responses, and intestinal barrier permeability. The current research indicates that the existence of DHA affects the improvement, DHA in phospholipid form could be a more effective choice for nutritional intervention to prevent and treat colitis.

Keywords: DHA-enriched phospholipids; colitis; gut microbiota; inflammation; intestinal barrier.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Oral
Animals
Colitis / chemically induced
Colitis / diet therapy*
Colitis / microbiology
Colitis / pathology
Cytokines / metabolism
Dextran Sulfate / toxicity
Dietary Supplements
Docosahexaenoic Acids / pharmacology
Encephalitis / diet therapy*
Encephalitis / etiology
Fatty Acids / analysis
Fatty Acids / chemistry
Fatty Acids, Omega-3 / chemistry
Fatty Acids, Omega-3 / pharmacology
Gastrointestinal Microbiome / drug effects*
Gastrointestinal Microbiome / genetics
Male
Mice
Mice, Inbred C57BL
Oxidative Stress / drug effects*
Phospholipids / administration & dosage
Phospholipids / pharmacology*

Substances

Cytokines
Fatty Acids
Fatty Acids, Omega-3
Phospholipids
Docosahexaenoic Acids
Dextran Sulfate