Bio-carrier-enhanced aerobic granulation: Effects on the extracellular polymeric substances production and microorganism community

Huihua Lin; Shichang Sun; Zipeng Lin; Manting Chen; Lin Fang; Rui Ma; Junhao Lin; Juan Luo

doi:10.1016/j.chemosphere.2021.130756

Bio-carrier-enhanced aerobic granulation: Effects on the extracellular polymeric substances production and microorganism community

Chemosphere. 2021 Oct:280:130756. doi: 10.1016/j.chemosphere.2021.130756. Epub 2021 May 3.

Authors

Huihua Lin¹, Shichang Sun², Zipeng Lin¹, Manting Chen¹, Lin Fang¹, Rui Ma³, Junhao Lin⁴, Juan Luo¹

Affiliations

¹ College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, China.
² College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, China; Research Center for Water Science and Environmental Engineering, Shenzhen University, 518055, China.
³ College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, China. Electronic address: marui067@163.com.
⁴ School of Environment, Harbin Institute of Technology, Harbin, 150090, China.

PMID: 33971405
DOI: 10.1016/j.chemosphere.2021.130756

Abstract

In this study, the strengthening effect of bio-carrier inoculation in the process of aerobic granulation and its influence on the microbial secretion of extracellular polymeric substances (EPS) has been systematically explored, to further understand and perfect the rapid granulation mechanism. Complete granulation was achieved within 15 days, and the granule morphology realized in a reactor inoculated with the bio-carrier (R1) was better than that in the control group (R2), in which complete granulation was not achieved during the entire operation period. However, AGS gradually disintegrated after the 20th day because of the strong shearing force, the crushed AGS enhanced granulation, however did not ensure stability. The average EPS content in R1 20 mg﹒gVSS^-1 higher than that in R2, and the protein (PN) content changes around 41.23-82.56 mg﹒gVSS^-1 during the granulation process. This indicates that the bio-carrier stimulates microorganisms to secrete more EPS, and PN may have a greater effect on the aggregation of microorganisms. The results showed that the addition of the bio-carrier shortened the AGS granulation time, and increased the EPS content, and the broken AGS played an auxiliary role as the nucleus for floc attachment.

Keywords: Bio-carrier; Extracellular polymeric substance; Granulation; Granulation mechanism; Microbiota.

MeSH terms

Aerobiosis
Bioreactors
Extracellular Polymeric Substance Matrix*
Sewage*
Waste Disposal, Fluid

Substances

Sewage