Grapes are known to synthesize resveratrol, a stilbene phytoalexin, associated with cancer chemopreventive activity and cardioprotection. The effect of ultrasound (US) abiotic elicitor treatments on trans-resveratrol content in Kalecik Karası fresh and frozen grape skin was determined. D-optimal point change design was used under RSM for the experimental design of US treatment. The optimization was solved with the help of the Pareto areas and the optimal input variable values were determined by the desirability function with fuzzy similar perceivable ratio method. The optimum conditions of US treatment for fresh grape skin were determined as follows: incubation time-24 h, US application method-(P01), US frequency-20 kHz, US treatment time-60 min and ultrasonic intensity (UI)-1.15 W cm-2. The trans-resveratrol content (0.18 ± 0.01 mg/g) in the untreated grape skin significantly increased with optimum US treatment (3.58 ± 0.08 mg/g), increasing production to 19.9 times.
Keywords: Abiotic elicitor; D-optimal design; Desirability function optimization; Grape skin; Trans-resveratrol; Ultrasound.
© Association of Food Scientists & Technologists (India) 2020.