CCL19 variants mediate chemotactic response via CCR7 in grass carp Ctenopharyngodon idella

Wei Wei; Junya Wang; Qingyu Min; Zhao Jia; Kangyong Chen; Hao Feng; Jun Zou

doi:10.1016/j.dci.2021.104127

CCL19 variants mediate chemotactic response via CCR7 in grass carp Ctenopharyngodon idella

Dev Comp Immunol. 2021 Sep:122:104127. doi: 10.1016/j.dci.2021.104127. Epub 2021 May 7.

Authors

Wei Wei¹, Junya Wang¹, Qingyu Min¹, Zhao Jia¹, Kangyong Chen¹, Hao Feng², Jun Zou³

Affiliations

¹ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
² State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, China.
³ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China. Electronic address: jzou@shou.edu.cn.

PMID: 33965447
DOI: 10.1016/j.dci.2021.104127

Abstract

CC chemokine ligand 19 (CCL19) plays a key role in the regulation of immune responses including homeostasis, inflammation, and immune tolerance. In this study, two variants of CCL19 homologues (CCL19a2 and CCL19b) and CCR7 were investigated in grass carp Ctenopharyngodon idella. The three genes were widely expressed in immune tissues and could be modulated by stimulation with LPS, PHA and poly(I:C), and infection with Flavobacterium columnare and grass carp reovirus. In an in vitro chemotaxis assay, the recombinant CCL19a2 and CCL19b were active to promote the migration of HEK293 T cells expressing CCR7 and leucocytes isolated from the gills, head kidney and spleen. Moreover, their chemotactive effects were validated in vivo. We found that the cells recruited by CCL19a2 and CCl19b are mainly monocytes/macrophages expressing high levels of IL-1β, IFN-γ, colony stimulating factor 1 receptor (CSF1R) and MHC II. Our work suggests that CCL19a2 and CCl19b are involved in recruitment of antigen presenting cells in fish.

Keywords: Bacterial and viral infection; Chemokine; Chemokine receptor; Chemotaxis; Gene expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigen Presentation / immunology*
Base Sequence
Carps / immunology*
Carps / microbiology
Cell Line
Cell Movement / immunology
Chemokine CCL19 / genetics
Chemokine CCL19 / immunology*
Fish Diseases / immunology*
Fish Diseases / microbiology
Flavobacterium / immunology
Gills / cytology
Gills / immunology
HEK293 Cells
Head Kidney / cytology
Head Kidney / immunology
Humans
Interferon-gamma / metabolism
Interleukin-1beta / metabolism
Leukocytes / immunology*
Lipopolysaccharides / pharmacology
Macrophages / immunology
Monocytes / immunology
Phytohemagglutinins / immunology
Poly I-C / immunology
Receptors, CCR7 / metabolism*
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
Reoviridae / immunology
Sequence Analysis, DNA
Spleen / cytology
Spleen / immunology

Substances

Chemokine CCL19
Interleukin-1beta
Lipopolysaccharides
Phytohemagglutinins
Receptors, CCR7
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Interferon-gamma
Poly I-C

Supplementary concepts

Flavobacterium columnare