An electrochemical aptasensor based on AuPt alloy nanoparticles for ultrasensitive detection of amyloid-β oligomers

Yibiao Liu; Qing Xu; Yina Zhang; Bingyu Ren; Liumei Huang; Hong Cai; Tailin Xu; Qiong Liu; Xueji Zhang

doi:10.1016/j.talanta.2021.122360

An electrochemical aptasensor based on AuPt alloy nanoparticles for ultrasensitive detection of amyloid-β oligomers

Talanta. 2021 Aug 15:231:122360. doi: 10.1016/j.talanta.2021.122360. Epub 2021 Mar 31.

Authors

Yibiao Liu¹, Qing Xu², Yina Zhang², Bingyu Ren³, Liumei Huang², Hong Cai⁴, Tailin Xu⁵, Qiong Liu⁶, Xueji Zhang⁷

Affiliations

¹ Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, China; College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, China.
² Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, China.
³ Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, China; Shenzhen Bay Laboratory, Shenzhen, 518055, China.
⁴ Department of Chemistry, Hanshan Normal University, Chaozhou, China.
⁵ Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, China. Electronic address: xutailin@szu.edu.cn.
⁶ Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, China; Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, China. Electronic address: liuqiong@szu.edu.cn.
⁷ Shenzhen Key Laboratory of Marine Biotechnology and Ecology, College of Life Sciences and Oceanography, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, Guangdong, 518060, China. Electronic address: zhangxueji@szu.edu.cn.

PMID: 33965026
DOI: 10.1016/j.talanta.2021.122360

Abstract

Amyloid-β oligomer is an important biomarker and a potential therapeutic target of Alzheimer's disease in its early stage. Here, we combined superhydrophobic carbon fiber paper (CFP) with AuPt alloy nanoparticles to prepare a CFP/AuPt nanocomposite with larger specific surface area and hydrophobic surface. On this basis, we constructed an electrochemical aptasensor based on CFP/AuPt for the ultrasensitive detection of amyloid-β oligomers. The surface-coated AuPt nanoparticles greatly enhanced the electroactive area, and the hydrophobic surface increased the resisting nonspecific adsorption performance of sensor. A combination of these two features significantly improved the sensitivity and specificity of the sensor. This electrochemical aptasensor based on CFP/AuPt displayed a low detection limit of 0.16 pg/mL. This work shows a promising future in clinical diagnosis of Alzheimer's disease and provides a possible solution to electrochemical biosensors that are susceptible to interference in biological fluids.

Keywords: Alzheimer's disease (AD); Amyloid-β oligomer; AuPt alloy Nanoparticles; Electrochemical aptasensor; Resisting nonspecific adsorption.

MeSH terms

Alloys
Amyloid beta-Peptides
Aptamers, Nucleotide*
Biosensing Techniques*
Electrochemical Techniques
Gold
Limit of Detection
Metal Nanoparticles*

Substances

Alloys
Amyloid beta-Peptides
Aptamers, Nucleotide
Gold