Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures

Hirotaka Watanabe; Rei Murakami; Hideyuki Okano

doi:10.1007/7651_2021_407

Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures

Methods Mol Biol. 2022:2549:209-217. doi: 10.1007/7651_2021_407.

Authors

Hirotaka Watanabe¹, Rei Murakami², Hideyuki Okano³

Affiliations

¹ Department of Physiology, Keio University, School of Medicine, Tokyo, Japan. hwatanabe@keio.jp.
² Department of Physiology, Keio University, School of Medicine, Tokyo, Japan.
³ Department of Physiology, Keio University, School of Medicine, Tokyo, Japan. hidokano@keio.jp.

PMID: 33959916
DOI: 10.1007/7651_2021_407

Abstract

Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.

Keywords: Amyloid β (Aβ); Enzyme-Linked Immuno Sorbent Assay (ELISA); Neuronal differentiation; induced pluripotent stem cell (iPSC); β-Secretase; γ-Secretase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alzheimer Disease* / pathology
Amyloid beta-Peptides
Cerebral Amyloid Angiopathy*
Enzyme-Linked Immunosorbent Assay
Humans
Induced Pluripotent Stem Cells* / pathology
Neurons / pathology

Substances

Amyloid beta-Peptides