Quantitative analysis of 20 fentanyl analogues by modified QuEChERS-LC-MS/MS in health products and transdermal patches

Abstract

The spreading of narcotics especially illicit novel psychoactive substances is a continuing problem in recent years. In response to reduce the morbidity and crime related to fentanyl analogues, the accurate measurement of fentanyl analogues concentrations is significantly important in the analytical laboratories for customs checks and clinical application. In this work, ethyl acetate was selected as extraction solvent, 50 mg of PSA, 100 mg of C18, and 10 mg of GCB were optimized for purification. A modified QuEChERS extraction method followed by high performance liquid chromatography-tandem mass spectrometry with the mode of multiple reaction monitoring has been developed for the simultaneous determination of 20 fentanyl analogues in collagen peptides, slimming capsules and fentanyl transdermal patches. The limits of detection (LODs) varied from 0.004 to 0.02 μg L^-1 with relative standard deviations of 4.89-11.4 % and showed good linearity in the range of 0.02-10 μg L^-1 and 0.01-1.00 mg L^-1, respectively. The recoveries for 20 fentanyl analogues in the low (at μg L^-1 level) and high (at mg L^-1 level) concentration spiked samples were in the range of 77.7-114 % and 83.9-116 %, which demonstrated the application potential of the proposed method for the determination of fentanyl analogues with low and high concentration in real case samples. In addition, the matrix effect and the cross-reactivity were also proved to not interfere with quantitation of targeted fentanyl analogues. Thus, the developed method showed high sensitivity and good accuracy, which makes it suitable for the rapid detection of fentanyl analogues for customs and border service as well as pharmaceuticals.

Keywords: Fentanyl analogues; Fentanyl transdermal patches; Health products; High performance liquid chromatography-tandem mass spectrometry; QuEChERS.