Natural soluble epoxide hydrolase inhibitors from Alisma orientale and their potential mechanism with soluble epoxide hydrolase

Int J Biol Macromol. 2021 Jul 31:183:811-817. doi: 10.1016/j.ijbiomac.2021.04.187. Epub 2021 May 3.

Abstract

Inhibition of soluble epoxide hydrolase (sEH) is considered to be an effective treatment for inflammation-related diseases, and small molecules origin from natural products show promising activity against sEH. Two undescribed protostanes, 3β-hydroxy-25-anhydro-alisol F (1) and 3β-hydroxy-alisol G (2) were isolated from Alisma orientale and identified as new sEH inhibitors with IC50 values of 10.06 and 30.45 μM, respectively. Potential lead compound 1 was determined as an uncompetitive inhibitor against sEH, which had a Ki value of 5.13 μM. In-depth molecular docking and molecular dynamics simulations revealed that amino acid residue Ser374 plays an important role in the inhibition of 1, which also provides an idea for the development of sEH inhibitors based on protostane-type triterpenoids.

Keywords: Inhibition kinetics; Molecular dynamics simulations; Soluble epoxide hydrolase.

MeSH terms

  • Alisma / chemistry*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology*
  • Epoxide Hydrolases / antagonists & inhibitors*
  • Epoxide Hydrolases / chemistry
  • Inhibitory Concentration 50
  • Models, Molecular
  • Molecular Docking Simulation
  • Molecular Dynamics Simulation
  • Molecular Structure
  • Pharmacokinetics
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Protein Conformation
  • Triterpenes / chemistry
  • Triterpenes / pharmacology*

Substances

  • Enzyme Inhibitors
  • Plant Extracts
  • Triterpenes
  • Epoxide Hydrolases