Laboratory Worker Self-Contamination with Noninfectious SARS-CoV-2 DNA Can Result in False-Positive Reverse Transcriptase PCR-Based Surveillance Testing
J Clin Microbiol
.
2021 Jun 18;59(7):e0072321.
doi: 10.1128/JCM.00723-21.
Epub 2021 Jun 18.
Authors
Theresa L Montgomery
1
,
Michelle Paavola
2
,
Emily A Bruce
3
4
,
Jason W Botten
3
4
5
,
Jessica W Crothers
4
6
,
Dimitry N Krementsov
1
Affiliations
1
Department of Biomedical and Health Sciences, College of Nursing and Health Sciences, University of Vermont, Burlington, Vermont, USA.
2
Center for Health and Wellbeing, University of Vermont, Burlington, Vermont, USA.
3
Department of Microbiology & Molecular Genetics, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
4
Translational Global Infectious Disease Research Center, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
5
Division of Immunobiology, Department of Medicine, Larner College of Medicine, University of Vermont, Burlington, Vermont, USA.
6
Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington, Vermont, USA.
PMID:
33952597
PMCID:
PMC8218757
DOI:
10.1128/JCM.00723-21
No abstract available
Keywords:
RT-PCR; SARS-CoV-2; cDNA; contamination; coronavirus; false positive.
Publication types
Letter
MeSH terms
COVID-19*
Clinical Laboratory Techniques
DNA
Humans
Laboratories
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2*
Substances
DNA
Grants and funding
P20 GM125498/GM/NIGMS NIH HHS/United States
T32 AI055402/AI/NIAID NIH HHS/United States