Bioactive glass activates VEGF paracrine signaling of cardiomyocytes to promote cardiac angiogenesis

Miao Shi; Fujian Zhao; Luyao Sun; Fengling Tang; Wendong Gao; Weihan Xie; Xiaodong Cao; Jian Zhuang; Xiaofeng Chen

doi:10.1016/j.msec.2021.112077

Bioactive glass activates VEGF paracrine signaling of cardiomyocytes to promote cardiac angiogenesis

Mater Sci Eng C Mater Biol Appl. 2021 May:124:112077. doi: 10.1016/j.msec.2021.112077. Epub 2021 Mar 26.

Authors

Miao Shi¹, Fujian Zhao², Luyao Sun², Fengling Tang², Wendong Gao², Weihan Xie², Xiaodong Cao², Jian Zhuang³, Xiaofeng Chen⁴

Affiliations

¹ School of Medicine, South China University of Technology, Guangzhou 510006, PR China; National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China; Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China.
² National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China; School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China; Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China.
³ School of Medicine, South China University of Technology, Guangzhou 510006, PR China; Guangdong General Hospital, Guangzhou 510080, PR China. Electronic address: zhuangjiangenetics@163.com.
⁴ National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China; School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, PR China; Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 510006, PR China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, PR China. Electronic address: chenxf@scut.edu.cn.

PMID: 33947569
DOI: 10.1016/j.msec.2021.112077

Abstract

The heart contains a wide range of cell types, which are not isolated but interact with one another via multifarious paracrine, autocrine and endocrine factors. In terms of cardiac angiogenesis, previous studies have proved that regulating the communication between cardiomyocytes and endothelial cells is efficacious to promote capillary formation. Firstly, this study investigated the effect and underlying mechanism of bioactive glass (BG) acted on vascular endothelial growth factor (VEGF) paracrine signaling in cardiomyocytes. We found that bioactive ions released from BG significantly promoted the VEGF production and secretion of cardiomyocytes. Subsequently, we proved that cardiomyocyte-derived VEGF played an important role in mediating the behavior of endothelial cells. Further research showed that the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/hypoxia-inducible factor 1α (HIF-1α) signaling pathway was upregulated by BG, which was involved in VEGF expression of cardiomyocytes. This study revealed that by means of modulating cellular crosstalk via paracrine signaling of host cells in heart is a new direction for the application of BGs in cardiac angiogenesis.

Keywords: Bioactive glass; Cardiac angiogenesis; Cellular crosstalk; Paracrine factor; VEGF.

MeSH terms

Endothelial Cells
Hypoxia-Inducible Factor 1, alpha Subunit
Myocytes, Cardiac*
Paracrine Communication
Phosphatidylinositol 3-Kinases
Vascular Endothelial Growth Factor A*

Substances

Hypoxia-Inducible Factor 1, alpha Subunit
Vascular Endothelial Growth Factor A