Roseomonas hellenica sp. nov., isolated from roots of wild-growing Alkanna tinctoria

Angélique Rat; Henry D Naranjo; Liesbeth Lebbe; Margo Cnockaert; Nikos Krigas; Katerina Grigoriadou; Eleni Maloupa; Anne Willems

doi:10.1016/j.syapm.2021.126206

Roseomonas hellenica sp. nov., isolated from roots of wild-growing Alkanna tinctoria

Syst Appl Microbiol. 2021 May;44(3):126206. doi: 10.1016/j.syapm.2021.126206. Epub 2021 Apr 17.

Authors

Angélique Rat¹, Henry D Naranjo¹, Liesbeth Lebbe¹, Margo Cnockaert¹, Nikos Krigas², Katerina Grigoriadou², Eleni Maloupa², Anne Willems¹

Affiliations

¹ Laboratory of Microbiology, Dept. Biochemistry and Microbiology, Fac. Sciences, Ghent University, Gent, Belgium.
² Laboratory of Conservation and Evaluation of Native and Floricultural Species, Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, Thessaloniki, Greece.

PMID: 33945925
DOI: 10.1016/j.syapm.2021.126206

Abstract

Two Gram-negative, aerobic, rod-shaped and yellow-orange pigmented bacterial strains (LMG 31523^T and LMG 31524) were isolated from roots of wild-growing Alkanna tinctoria plants collected near Thessaloniki, Greece. Analysis of their 16S rRNA gene sequences revealed that they form a separate cluster related to the genus Roseomonas. A comparative whole genome analysis of the two strains and the type strains of related Roseomonas species revealed average nucleotide identity values from 78.84 and 80.32%. The G + C contents of the genomic DNA of strains LMG 31523^T and LMG 31524 were 69.69% and 69.74%, respectively. Combined data from phenotypic, phylogenetic and chemotaxonomic studies indicated that the strains LMG 31523^T and LMG 31524 represent a novel species of the genus Roseomonas. Genome analysis of the new strains showed a number of genes involved in survival in the rhizosphere environment and in plant colonization and confirmed the endophytic characteristics of LMG 31523^T and LMG 31524. Since the strains LMG 31523^T and LMG 31524 were isolated from a plant collected in Greece the name Roseomonas hellenica sp. nov. is proposed. The type strain is LMG 31523^T (=CECT 30032^T).

Keywords: Endophytic bacteria; Genome analysis; Greece; Medicinal plant; New species; Red alkanet.

MeSH terms

Bacterial Typing Techniques
Base Composition
Boraginaceae* / microbiology
DNA, Bacterial / genetics
Endophytes
Greece
Methylobacteriaceae* / classification
Methylobacteriaceae* / isolation & purification
Phylogeny*
Pigmentation
Plant Roots / microbiology
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA

Substances

DNA, Bacterial
RNA, Ribosomal, 16S