Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research

Chuanxin Chen; Weizhi Ji; Yuyu Niu

doi:10.1016/j.tibtech.2021.03.010

Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research

Trends Biotechnol. 2021 Dec;39(12):1332-1342. doi: 10.1016/j.tibtech.2021.03.010. Epub 2021 Apr 30.

Authors

Chuanxin Chen¹, Weizhi Ji², Yuyu Niu³

Affiliations

¹ State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Yunnan, Kunming, China.
² State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Yunnan, Kunming, China. Electronic address: wji@lpbr.cn.
³ State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Yunnan, Kunming, China; Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan, Kunming, China. Electronic address: niuyy@lpbr.cn.

PMID: 33941418
DOI: 10.1016/j.tibtech.2021.03.010

Abstract

The improved ability to organize pluripotent stem cells (PSCs) into 3D structures in vitro has shed light on organoid technology to recapitulate organs and tumors in vivo. Advances in gene-editing technologies, particularly CRISPR-mediated techniques, offer tremendous potential in facilitating organoid research, including the study of development, disease modeling, and personalized medicine. This review discusses how the combination of two novel technologies - organoids and gene editing - not only contributes to revealing molecular events taking place during development and tumorigenesis but also has implications for biobanking, precision medicine, and other diverse biomedical applications.

Keywords: CRISPR; gene editing; non-human primate; organoid; stem cell.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Biological Specimen Banks
Biomedical Research*
Gene Editing / methods
Organoids*
Primates
Technology