LncRNA ENSG00000254615 Modulates Proliferation and 5-FU Resistance by Regulating p21 and Cyclin D1 in Colorectal Cancer

Yang Fu; Runqing Huang; Jianxia Li; Xiaoyu Xie; Yanhong Deng

doi:10.1080/07357907.2021.1923727

LncRNA ENSG00000254615 Modulates Proliferation and 5-FU Resistance by Regulating p21 and Cyclin D1 in Colorectal Cancer

Cancer Invest. 2021 Oct;39(9):696-710. doi: 10.1080/07357907.2021.1923727. Epub 2021 Sep 1.

Authors

Yang Fu^{1

2}, Runqing Huang^{1

2

3}, Jianxia Li^{1

2}, Xiaoyu Xie¹, Yanhong Deng^{1

2

3}

Affiliations

¹ Department of Medical Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.
² Guangdong Research Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.
³ Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.

PMID: 33938344
DOI: 10.1080/07357907.2021.1923727

Abstract

5-Fluorouracil (5-FU) resistance is an urgent problem of colorectal cancer (CRC) chemotherapy that needs to be resolved. To investigate 5-FU-associated lncRNAs for CRC might be of great significant. LncRNA ENSG00000254615 was detected by RNA-sequencing. ENSG00000254615 were detected highly expressed in 5-FU-sensitive CRC cells and tissue specimens, and inhibited cell proliferation and attenuated 5-FU resistance in vitro and in vivo. Furthermore, ENSG00000254615 participated in the regulation of p21 and Cyclin D1. Taken together, we proposed that ENSG00000254615 inhibits proliferation and attenuates 5-FU resistance of CRC by regulating p21 and Cyclin D1 expression.

Keywords: 5-FU resistance; Colorectal cancer; lncRNA; proliferation.

MeSH terms

Animals
Antimetabolites, Antineoplastic / pharmacology
Cell Line, Tumor
Cell Proliferation / genetics*
Colorectal Neoplasms / drug therapy
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / metabolism
Cyclin D1 / genetics*
Cyclin D1 / metabolism
Cyclin-Dependent Kinase Inhibitor p21 / genetics*
Cyclin-Dependent Kinase Inhibitor p21 / metabolism
Drug Resistance, Neoplasm / genetics*
Fluorouracil / pharmacology*
Gene Expression Regulation, Neoplastic*
HCT116 Cells
HEK293 Cells
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
RNA, Long Noncoding / genetics*
Tumor Burden / drug effects
Tumor Burden / genetics
Xenograft Model Antitumor Assays / methods

Substances

Antimetabolites, Antineoplastic
CDKN1A protein, human
Cyclin-Dependent Kinase Inhibitor p21
RNA, Long Noncoding
Cyclin D1
Fluorouracil