Carbonic Anhydrase in Pacific Abalone Haliotis discus hannai: Characterization, Expression, and Role in Biomineralization

Md Rajib Sharker; Soo Cheol Kim; Shaharior Hossen; Kanij Rukshana Sumi; Sang Ki Choi; Kap Seong Choi; Kang Hee Kho

doi:10.3389/fmolb.2021.655115

Carbonic Anhydrase in Pacific Abalone Haliotis discus hannai: Characterization, Expression, and Role in Biomineralization

Front Mol Biosci. 2021 Apr 15:8:655115. doi: 10.3389/fmolb.2021.655115. eCollection 2021.

Authors

Md Rajib Sharker^{1

2}, Soo Cheol Kim¹, Shaharior Hossen¹, Kanij Rukshana Sumi³, Sang Ki Choi⁴, Kap Seong Choi⁵, Kang Hee Kho¹

Affiliations

¹ Department of Fisheries Science, College of Fisheries and Ocean Sciences, Chonnam National University, Yeosu, South Korea.
² Department of Fisheries Biology and Genetics, Faculty of Fisheries, Patuakhali Science and Technology University, Patuakhali, Bangladesh.
³ Department of Aquaculture, Faculty of Fisheries, Patuakhali Science and Technology University, Patuakhali, Bangladesh.
⁴ Department of Biological Sciences, College of Life Industry and Science, Sunchon National University, Suncheon, South Korea.
⁵ Department of Food Science and Technology, Sunchon National University, Suncheon, South Korea.

Abstract

Carbonic anhydrases (CAs) are universal zinc ion containing metalloenzymes that play a pivotal role in various physiological processes. In this study, a CA I (designated as Hdh CA I) was isolated and characterized from the mantle tissue of Pacific abalone, Haliotis discus hannai. The full-length cDNA sequence of Hdh CA I was 1,417-bp in length, encoding a protein of 337 amino acids with molecular weight of 37.58 kDa. Hdh CA I sequence possessed a putative signal peptide of 22 amino acids and a CA catalytic function domain. The predicted protein shared 94 and 78% sequence identities with Haliotis gigantea and Haliotis tuberculata CA I, respectively. Results of phylogenetic analysis indicated that Hdh CA I was evolutionarily close to CA I of H. gigantea and H. tuberculata with high bootstrap values. Significantly higher levels of Hdh CA I mRNA transcript were found in mantle than other examined tissues. In situ hybridization results showed strong hybridization signals in epithelial cells of the dorsal mantle pallial, an area known to synthesize and secrete proteins responsible for the nacreous layer formation of shell. This is the first study on Hdh CA I in H. discus hannai and the results may contribute to further study its physiological functions in shell biomineralization of abalone.

Keywords: Haliotis discus hannai; biomineralization; carbonic anhydrase; in situ hybridization; qRT-PCR.