O-polysaccharide (O-antigen, OPS) was isolated from the lipopolysaccharide of Pseudomonas veronii SHC-8-1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: where QuipNAc4N(dHh) is 2,4-diamino-2,4,6-trideoxy-dglucose (Bacillosamine) in which N-2 is acetylated and N-4 is acylated with 3,5-dihydroxyhexanoic acid (dHh). The O-antigen gene cluster of Pseudomonas veronii SHC-8-1 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the OPS structure.
Keywords: Bacterial polysaccharide structure; Lipopolysaccharide; O-antigen; O-antigen gene cluster; O-specific polysaccharide; Pseudomonas veronii.
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