Bioassay-guided isolation and characterization of antibacterial compound from Aspergillus fumigatus HX-1 associated with Clam

Xiaowen Xu; Siya Guo; Hui Chen; Zongyi Zhang; Xiangrong Li; Wenbin Wang; Lei Guo

doi:10.1007/s13205-021-02754-3

Bioassay-guided isolation and characterization of antibacterial compound from Aspergillus fumigatus HX-1 associated with Clam

3 Biotech. 2021 Apr;11(4):193. doi: 10.1007/s13205-021-02754-3. Epub 2021 Mar 25.

Authors

Xiaowen Xu^{1

2

3}, Siya Guo^{1

2

3}, Hui Chen^{1

2

3}, Zongyi Zhang^{1

2

3}, Xiangrong Li^{1

2

3}, Wenbin Wang^{1

2

3}, Lei Guo^{1

2

3}

Affiliations

¹ Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, 222005 China.
² Jiangsu Key Laboratory of Marine Biotechnology, School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang, 222005 China.
³ Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, School of Pharmacy, Jiangsu Ocean University, Lianyungang, 222005 China.

Abstract

This study aimed to identify a symbiotic fungus strain HX-1 with anti-Vibrio harveyi activity and isolate and identify the active compound. The HX-1 strain was identified as Aspergillus fumigatus according to the morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The compound was isolated from the fermentation product of HX-1 strain through ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatography, and semi-preparative HPLC techniques using an antibacterial-guided fractionation method. According to its physicochemical properties and spectral characteristics, the compound was identified as trypacidin having the same anti-V. harveyi activity as streptomycin sulfate, with the minimum inhibitory concentration of 31.25 µg/mL.

Keywords: Antibacterial activity; Aspergillus fumigatus; Bioassay-guided isolation; Trypacidin.