Primary mouse osteoblast and osteoclast culturing and analysis

Claire Chevalier; Melis Çolakoğlu; Julia Brun; Cyril Thouverey; Nicolas Bonnet; Serge Ferrari; Mirko Trajkovski

doi:10.1016/j.xpro.2021.100452

Primary mouse osteoblast and osteoclast culturing and analysis

STAR Protoc. 2021 Apr 13;2(2):100452. doi: 10.1016/j.xpro.2021.100452. eCollection 2021 Jun 18.

Authors

Claire Chevalier^{1

2}, Melis Çolakoğlu^{1

2}, Julia Brun³, Cyril Thouverey³, Nicolas Bonnet^{2

4}, Serge Ferrari^{2

3}, Mirko Trajkovski^{1

2}

Affiliations

¹ Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
² Diabetes Center, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
³ Division of Bone Diseases, Geneva University Hospitals, 1211 Geneva, Switzerland.
⁴ Nestle Research, Innovation EPFL Park, 1015 Lausanne, Switzerland.

Abstract

Mesenchymal-derived osteoblasts play a key role in bone formation via synthesis and mineralization of the bone and bone remodeling. Osteoclasts are multinucleated cells of hematopoietic origin with a role in bone resorption. Here, we describe a protocol for generating primary cultures of these two cell types from bone tissue including the femur, tibia, and humerus of young mice. We describe methods for addressing their activity and/or differentiation, enabling studying the effects of various treatments during or following differentiation ex vivo. For further practical example of using these protocols, please refer to Chevalier et al. (2020).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Cell isolation; Metabolism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Mice
Osteoblasts / cytology*
Osteoblasts / metabolism*
Osteoclasts / cytology*
Osteoclasts / metabolism*
Primary Cell Culture*