Potassium modulates central carbon metabolism to participate in regulating CO2 transport and assimilation in Brassica napus leaves

Abstract

Potassium (K) regulates plant metabolism and enhances plant's ability to adapt to adversity. However, under different K deficiency stress, the net photosynthetic rate (A_n) was reduced, influenced by CO₂ conductance or biochemical capacities. The interplay between metabolome and photosynthetic characteristics under K deficiency stress was analyzed to explore the mechanisms by which K regulates photosynthetic capacity. With increasing K deficiency stress, dominations limiting A_n varied from CO₂ conductance to biochemical limitations. Multivariate analyses indicated that organic acids, amino acids and sedoheptulose-7-bisphosphate were significantly related to A_n, CO₂ conductance and carboxylation rate. Under moderate K deficiency, organic acids were up-regulated. Acidification of subcellular compartments reduced sedoheptulose-1,7-bisphosphatase activity, inducing downregulation of sedoheptulose-7-bisphosphate and hindrance of ribulose bisphosphate regeneration. Moreover, increased CO₂ shortage with increasing K deficiency induced a shift of increased citric acid to amino acid synthesis, causing excessive accumulation of amino acids. In addition, the reduced serine level indicated impaired photorespiration. These two changes triggered more serious reduction in photosynthetic capacity. The intimate, changes in photosynthetic capacities were tightly coupled with shifts in central C metabolism, which provides insights into the methods used to enhance A_n and plant's adaptability to abiotic stresses, through the regulation of C metabolites using molecular technology.

Keywords: Amino acid; Central carbon metabolism; Photosynthetic characteristics; Potassium; Sedoheptulose-7-bisphosphate.