Two ACTH analogs exert differential effects on monocytes/macrophages function regulation in ayu (Plecoglossus altivelis)

Lin-Lin Feng; You-Wu Dai; Xin-Jiang Lu; Jian-Fei Lu; Guan-Jun Yang; Hao Zhang; Li Zhang; Jiong Chen

doi:10.1016/j.ygcen.2021.113796

Two ACTH analogs exert differential effects on monocytes/macrophages function regulation in ayu (Plecoglossus altivelis)

Gen Comp Endocrinol. 2022 Jan 1:315:113796. doi: 10.1016/j.ygcen.2021.113796. Epub 2021 Apr 24.

Authors

Lin-Lin Feng¹, You-Wu Dai¹, Xin-Jiang Lu², Jian-Fei Lu¹, Guan-Jun Yang¹, Hao Zhang¹, Li Zhang¹, Jiong Chen³

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), China. Electronic address: lxj711043@163.com.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. Electronic address: jchen1975@163.com.

PMID: 33901496
DOI: 10.1016/j.ygcen.2021.113796

Abstract

Adrenocorticotropic hormone (ACTH), a bioactive peptide of the family of melanocortins, is generated from pro-opiomelanocortin (POMC). So far, the research on the specific functions of ACTH in the immune system of teleosts is limited. We determined two complementary DNA (cDNA) sequences of POMC in ayu (Plecoglossus altivelis), termed PaPOMC-A and PaPOMC-B. PaPOMCs transcripts occurred in all examined tissues, and their expression in immune tissues changed following experimental infection with Vibrio anguillarum. PaACTH-B, but not PaACTH-A, suppressed the phagocytosis of monocytes/macrophages (MO/MФ). Two isoforms of PaACTH increased the bactericidal capacity of MO/MФ. PaACTH-A increased anti-inflammatory cytokine expression, while PaACTH-B decreased pro-inflammatory cytokine expression in MO/MФ. Compared with PaACTH-B treatment, the PaACTH-A treatment improved survival rate and reduced the bacterial load in V. anguillarum-infected ayu through interleukin (IL)-10. Our results indicate that the two PaACTH isoforms exert different effects in the host defense against bacterial infection.

Keywords: Adrenocorticotropic hormone; Anti-inflammation; Ayu; Monocytes/macrophages; Phagocytosis; Pro-opiomelanocortin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adrenocorticotropic Hormone / metabolism
Adrenocorticotropic Hormone / pharmacology
Animals
Fish Diseases* / genetics
Fish Proteins / genetics
Fish Proteins / metabolism
Macrophages / metabolism
Macrophages / microbiology
Monocytes / metabolism
Monocytes / microbiology
Osmeriformes* / genetics
Osmeriformes* / metabolism
Vibrio Infections* / genetics
Vibrio Infections* / microbiology
Vibrio*

Substances

Fish Proteins
Adrenocorticotropic Hormone