How to Activate Heat-Responsible Retrotransposon ONSEN in Brassicaceae Species

Hidetaka Ito

doi:10.1007/978-1-0716-1134-0_18

How to Activate Heat-Responsible Retrotransposon ONSEN in Brassicaceae Species

Methods Mol Biol. 2021:2250:189-194. doi: 10.1007/978-1-0716-1134-0_18.

Author

Hidetaka Ito¹

Affiliation

¹ Faculty of Science, Hokkaido University, Sapporo, Hokkaido, Japan. hito@sci.hokudai.ac.jp.

PMID: 33900605
DOI: 10.1007/978-1-0716-1134-0_18

Abstract

A number of transposable elements are activated by environmental stress. A Ty1/copia-type retrotransposon named ONSEN is activated by heat stress in Brassicaceae species. A synthetic activation of the transposon is effective for the molecular breeding without genetic modification. Here, we described the detail procedure of heat treatment to activate ONSEN in Brassicaceae species.

Keywords: Arabidopsis; Brassicaceae; Heat stress; ONSEN; Retrotransposon.

MeSH terms

Arabidopsis / genetics
Arabidopsis / growth & development*
DNA, Plant / genetics
Gene Expression Regulation, Plant
Heat-Shock Response
Plant Breeding
Retroelements*

Substances

DNA, Plant
Retroelements