Unhealthy diet, habits and drug abuse cause a variety of liver diseases, including steatohepatitis, liver fibrosis, liver cirrhosis and liver cancer, which seriously affect human health. The fabrication of highly simulated cell models in vitro is important in the treatment of liver diseases and drug development. This article summarized the common strategies for the construction of liver pathology models in vitro. It introduced four typical cell models in vitro related to liver disease and provided a reference for the study of liver disease models.
不健康的饮食、作息和滥用药物会引起多种肝脏疾病，包括脂肪性肝炎、肝纤维化、肝硬化和肝癌等，严重影响人体健康。构筑体外细胞模型在研究肝病及其药物开发中具有重要意义。目前，国内外研究者已经开发出多种体外肝脏疾病模型。本文概括了体外肝脏病理模型构建的常用策略，并从原料、方法、指标等方面介绍了四种典型肝病相关的体外细胞模型，期望可为肝病模型相关研究人员提供参考。.
Keywords: liver diseases; liver function; microenvironment; model in vitro; protein biomarkers.