Microbiome analysis reveals the alterations in gut microbiota in different intestinal segments of Yimeng black goats

Aoyun Li; Yan Yang; Yan Zhang; Shenjin Lv; Taihua Jin; Kun Li; Zhaoqing Han; Yongzhu Li

doi:10.1016/j.micpath.2021.104900

Microbiome analysis reveals the alterations in gut microbiota in different intestinal segments of Yimeng black goats

Microb Pathog. 2021 Jun:155:104900. doi: 10.1016/j.micpath.2021.104900. Epub 2021 Apr 21.

Authors

Aoyun Li¹, Yan Yang², Yan Zhang³, Shenjin Lv⁴, Taihua Jin⁴, Kun Li⁵, Zhaoqing Han⁶, Yongzhu Li⁷

Affiliations

¹ College of Agriculture and Forestry Science, Linyi University, Linyi, 276000, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
² Linyi Academy of Agricultural Sciences, Linyi, 276012, China.
³ College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
⁴ College of Agriculture and Forestry Science, Linyi University, Linyi, 276000, China.
⁵ Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: lk3005@njau.edu.cn.
⁶ College of Agriculture and Forestry Science, Linyi University, Linyi, 276000, China. Electronic address: hanzhaoqing@lyu.edu.cn.
⁷ College of Agriculture and Forestry Science, Linyi University, Linyi, 276000, China. Electronic address: liyongzhu@lyu.edu.cn.

PMID: 33894292
DOI: 10.1016/j.micpath.2021.104900

Abstract

Mounting evidence revealed the importance of gut microbiota in host metabolism, immunity and physiology, and health. Yimeng black goats (YBGs) mainly distributed in Shandong province of China, displayed a complicated intestinal microecosystem, but studies of its gut microbiota are still insufficient to report. Therefore, this study was performed with an objective to characterize the intestinal microbial community structure and diversity in the small intestine (duodenum, jejunum and ileum) and cecum of YBGs and investigated the variability of gut microbiota of different intestinal segments. A total of 12 intestinal samples were collected from YBGs for high-throughput sequencing analysis based on V3-V4 variable region of 16S rRNA genes. Our results revealed alterations in gut microbial composition with obvious differences in relative abundance between the different intestinal segments. Additionally, small intestine including duodenum, jejunum and ileum not only displayed higher species abundance and diversity than cecum but also showed a significant difference among the main components of gut microbiota based on the analytical results of alpha and beta diversities. At the phylum level, Firmicutes and Proteobacteria were the most preponderant phyla in all the samples regardless of intestinal sites. Moreover, the microbiota in small intestine was significantly different from cecum, which were characterized by the higher relative abundance of Butyrivibrio_2, Megasphaera, Halomonas, Delftia, Hydrogenophaga, Limnobacter, Pseudoxanthomonas, Novosphingobium, Janibacter and Erythrobacter, whereas the levels of Butyricicoccus, unidentified_Lachnospiraceae, Fusicatenibacter, Akkermansia, Ruminococcaceae_NK4A214_group and Lactobacillus were lower. Overall, this study first characterized the profile of gut microbiota composition in different intestinal sites and provide better insight into intestinal microbial community structure and diversity of YBGs.

Keywords: 16S rRNA; Gut microbiota; High-throughput sequencing; Yimeng black goats.

MeSH terms

Animals
China
Gastrointestinal Microbiome*
Goats
RNA, Ribosomal, 16S / genetics

Substances

RNA, Ribosomal, 16S