The role of the neuropeptide [His7]-corazonin on phase-related characteristics in the Central American locust

Abstract

Density-dependent phase polyphenism in locusts is one of the most extreme forms of phenotypic plasticity. Locusts exist along the continuum between two density-dependent phenotypes that differ in nymphal coloration, behavior, morphology, physiology, and reproduction among others. Nymphs of the solitarious phase, found in low population densities, are usually green, relatively inactive, and avoid each other, while gregarious nymphs, found in high density, exhibit a very obvious yellow/orange background with black patterning, and are highly active and attracted to each other. The multifunctional neuropeptide [His⁷]-corazonin has been shown to strongly affect black coloration and several other phase-related characteristics in at least two locust species, even though no effect on phase-related behavioral traits has been found. In this study, we investigate the role of [His⁷]-corazonin in the Central American locust Schistocerca piceifrons (Walker), which evolved density-dependent phase polyphenism independently from the two previously studied locust species. After successfully knocking down the transcript encoding [His⁷]-corazonin (CRZ) using RNA interference, we show that such a knockdown influences both color and morphometrics in this species, but does not influence phase-related behavioral traits. Our results suggest that the role of [His⁷]-corazonin is conserved in different locust species. Finally, our study represents the first controlled study of behavioral solitarization in S. piceifrons.

Keywords: Behavior; Color; Morphology; Phenotypic plasticity.