Genome and transcriptome of a pathogenic yeast, Candida nivariensis

G3 (Bethesda). 2021 Jul 14;11(7):jkab137. doi: 10.1093/g3journal/jkab137.

Abstract

We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

Keywords: Candida nivariensis; genome assembly; nanopore sequencing; pathogenic yeast; transcriptome.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Genome
  • RNA
  • Saccharomyces cerevisiae*
  • Sequence Analysis, DNA
  • Transcriptome*

Substances

  • RNA

Supplementary concepts

  • Candida nivariensis