Whole-Genome Sequencing and De Novo Assembly of 61 Staphylococcus pseudintermedius Isolates from Healthy Dogs and Dogs with Pyoderma

O Francino; D Pérez; J Viñes; R Fonticoba; S Madroñero; G Meroni; P Martino; S Martínez; A Cusco; N Fàbregas; L Migura-García; L Ferrer

doi:10.1128/MRA.00152-21

Whole-Genome Sequencing and De Novo Assembly of 61 Staphylococcus pseudintermedius Isolates from Healthy Dogs and Dogs with Pyoderma

Microbiol Resour Announc. 2021 Apr 22;10(16):e00152-21. doi: 10.1128/MRA.00152-21.

Authors

O Francino¹, D Pérez², J Viñes^{2

3}, R Fonticoba⁴, S Madroñero², G Meroni⁵, P Martino⁵, S Martínez⁶, A Cusco³, N Fàbregas³, L Migura-García⁷, L Ferrer^{4

8}

Affiliations

¹ SVGM, Molecular Genetics Veterinary Service, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain olga.francino@uab.cat.
² SVGM, Molecular Genetics Veterinary Service, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
³ Vetgenomics, Edifici EUREKA, PRUAB, Bellaterra, Barcelona, Spain.
⁴ Department of Animal Medicine and Surgery, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.
⁵ Department of Biomedical, Surgical and Dental Sciences-One Health Unit, Milan, Italy.
⁶ Hospital Escuela de Pequeños Animales (HEPA), Facultad de Ciencias Veterinarias de la Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Buenos Aires, Argentina.
⁷ IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra, Barcelona, Spain.
⁸ Animal Medicine and Surgery, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, Spain.

Abstract

We have de novo assembled and polished 61 Staphylococcus pseudintermedius genome sequences with Nanopore-only long reads. Completeness was 99.25%. The average genome size was 2.70 Mbp, comprising 2,506 coding sequences, 19 complete rRNAs, 56 to 59 tRNAs, and 4 noncoding RNAs (ncRNAs), as well as CRISPR arrays.