Protective effect of scorpion venom heat-resistant synthetic peptide against PM2.5-induced microglial polarization via TLR4-mediated autophagy activating PI3K/AKT/NF-κB signaling pathway

J Neuroimmunol. 2021 Jun 15:355:577567. doi: 10.1016/j.jneuroim.2021.577567. Epub 2021 Apr 2.

Abstract

There is growing evidence that fine particulate matter (PM2.5) is a considerable risk factor for neurodegenerative diseases. Scorpion venom heat-resistant synthetic peptide (SVHRSP) plays a neuroprotective effect by promoting neurogenesis and neuron axon growth. In this study, SVHRSP inhibited the level of TLR4, autophagy and PM2.5-induced microglia M1 polarization, thereby promoting Phosphorylation of PI3K and AKT, inhibiting the expression of NF-κB. Moreover, SVHRSP suppressed the cytotoxic factors and increased the cytoprotective factor. This research demonstrates that SVHRSP relieves PM2.5-induced microglial polarization via TLR4-mediated autophagy activating PI3K/AKT/NF-κB signaling pathway, which provides new insights for the treatment of PM2.5-induced neurodegenerative diseases.

Keywords: Autophagy; PI3K/AKT/NF-κB pathway; PM(2.5); Polarization; Scorpion venom heat-resistant synthetic peptide; TLR4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autophagy / drug effects
  • Autophagy / physiology
  • Cell Line
  • Cell Polarity / drug effects
  • Cell Polarity / physiology
  • Cytoprotection / drug effects
  • Cytoprotection / physiology
  • Mice
  • Microglia / drug effects
  • Microglia / metabolism*
  • NF-kappa B / metabolism*
  • Particulate Matter / toxicity*
  • Phosphatidylinositol 3-Kinases / metabolism*
  • Proto-Oncogene Proteins c-akt / metabolism*
  • Scorpion Venoms / pharmacology*

Substances

  • NF-kappa B
  • Particulate Matter
  • Scorpion Venoms
  • Proto-Oncogene Proteins c-akt