Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging

Yingjie Yu; Lidan Li; Guobao Li; Xu Zhou; Tingting Deng; Meng Liang; Guohui Nie

doi:10.1039/d1cc00536g

Intracellular enzyme-powered DNA circuit with a tunable amplifier for miRNA imaging

Chem Commun (Camb). 2021 Apr 21;57(31):3753-3756. doi: 10.1039/d1cc00536g. Epub 2021 Mar 22.

Authors

Yingjie Yu¹, Lidan Li, Guobao Li, Xu Zhou, Tingting Deng, Meng Liang, Guohui Nie

Affiliation

¹ Institute of Translational Medicine, Department of Otolaryngology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen 518035, China. nieguohui@email.szu.edu.cn.

PMID: 33876121
DOI: 10.1039/d1cc00536g

Abstract

We describe an intracellular enzyme-powered DNA circuit probe with a tunable amplifier for sensitive and selective detection of miRNA. This approach has been successfully applied for in situ miRNA-21 fluorescence imaging in live cells. Also, we used chemicals to elevate the APE1 expression level rendering a tunable amplification strength for more flexible imaging applications.

MeSH terms

A549 Cells
DNA / chemistry*
DNA / metabolism
DNA Probes / chemistry*
DNA Probes / metabolism
DNA-(Apurinic or Apyrimidinic Site) Lyase / chemistry*
DNA-(Apurinic or Apyrimidinic Site) Lyase / metabolism
Humans
MCF-7 Cells
MicroRNAs / analysis*
MicroRNAs / metabolism
Optical Imaging

Substances

DNA Probes
MicroRNAs
DNA
APEX1 protein, human
DNA-(Apurinic or Apyrimidinic Site) Lyase