[Usefulness of the Sensititre YeastOne® panel to detect Candida species resistant to antifungal drugs]

Abstract

We evaluated the interlaboratory agreement, the essential agreement, and the categorical agreement between the Sensititre YeastOne^TM panel and the reference methods M27 4^th Edition of the Clinical and Laboratory Standards Institute (CLSI), and the EDef 7.3.1 of the European Committee on Antifungal Susceptibility Testing (EUCAST). We studied 67 Candida strains isolated from different clinical samples and 9 Candida strains with resistance to fluconazole and echinocandins. The highest percentage of interlaboratory agreement was observed with amphotericin B (96.8%), and the lowest percentage with voriconazole (77.2%). Caspofungin showed 5.8% of very major errors when compared with the CLSI reference method. For EUCAST, itraconazole, posaconazole, and anidulafungin showed high percentages of major errors: 17.6%, 18.1%, and 19.6%, respectively. Sensititre YeastOne^TM is a reliable alternative, and easy to perform for detecting Candida species resistant to antifungal drugs, with some limitations for echinocandins. Results are comparable to those of the reference methods.

Keywords: Antifungal; Antifúngicos; Candida spp.; Resistance; Resistencia; Sensititre.