Purification of differentiated tumor cells from medulloblastoma for transplantation into mouse cerebellum

Yijun Yang; Yanghui Qu; Yan Cheng; Duancheng Guo; Qianhai Fan; Zeng-Jie Yang

doi:10.1016/j.xpro.2021.100409

Purification of differentiated tumor cells from medulloblastoma for transplantation into mouse cerebellum

STAR Protoc. 2021 Mar 26;2(2):100409. doi: 10.1016/j.xpro.2021.100409. eCollection 2021 Jun 18.

Authors

Yijun Yang¹, Yanghui Qu², Yan Cheng¹, Duancheng Guo^{1

2}, Qianhai Fan^{1

2}, Zeng-Jie Yang¹

Affiliations

¹ Cancer biology program, Fox Chase Cancer Center, Temple University Health System, Philadelphia, PA, USA.
² Pediatric Cancer Center, College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, China.

Abstract

This protocol provides the procedures for isolating differentiated tumor cells from medulloblastoma (MB) in mice. Procedures for transplantation into cerebella are also included to examine the tumorigenesis of differentiated MB cells. This protocol outlines the detailed steps required for (1) isolation of tumor cells from mouse MB, (2) purification of differentiated tumor cells by fluorescence-activated cell sorting, and (3) transplantation of tumor cells into cerebella. This protocol is useful to purify differentiated tumor cells for investigating mechanisms underlying MB progression. For complete details on the use and execution of this protocol, please refer to Cheng et. al. (2020).

Keywords: Cancer; Cell isolation; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods
Cell Differentiation
Cell Separation / methods*
Cell Transplantation
Cerebellar Neoplasms / pathology*
Cerebellum / surgery*
Medulloblastoma / pathology*
Mice
Tumor Cells, Cultured* / cytology
Tumor Cells, Cultured* / pathology
Tumor Cells, Cultured* / transplantation