Comparing a Mixed Model Approach to Traditional Stability Estimators for Mapping Genotype by Environment Interactions and Yield Stability in Soybean [ Glycine max (L.) Merr.]

Mary M Happ; George L Graef; Haichuan Wang; Reka Howard; Luis Posadas; David L Hyten

doi:10.3389/fpls.2021.630175

Comparing a Mixed Model Approach to Traditional Stability Estimators for Mapping Genotype by Environment Interactions and Yield Stability in Soybean [ Glycine max (L.) Merr.]

Front Plant Sci. 2021 Mar 31:12:630175. doi: 10.3389/fpls.2021.630175. eCollection 2021.

Authors

Mary M Happ¹, George L Graef¹, Haichuan Wang¹, Reka Howard², Luis Posadas¹, David L Hyten¹

Affiliations

¹ Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, United States.
² Department of Statistics, University of Nebraska-Lincoln, Lincoln, NE, United States.

Abstract

Identifying genetic loci associated with yield stability has helped plant breeders and geneticists begin to understand the role and influence of genotype by environment (GxE) interactions in soybean [Glycine max (L.) Merr.] productivity, as well as other crops. Quantifying a genotype's range of performance across testing locations has been developed over decades with dozens of methodologies available. This includes directly modeling GxE interactions as part of an overall model for yield, as well as methods which generate overall yield "stability" values from multi-environment trial data. Correspondence between these methods as it pertains to the outcomes of genome wide association studies (GWAS) has not been well defined. In this study, the GWAS results for yield and yield stability were compared in 213 soybean lines across 11 environments to determine their utility and potential intersection. Both univariate and multivariate conventional stability estimates were considered alongside a mixed model for yield that fit marker by environment interactions as a random effect. One-hundred and six total QTL were discovered across all mapping results, however, genetic loci that were significant in the mixed model for grain yield that fit marker by environment interactions were completely distinct from those that were significant when mapping using traditional stability measures as a phenotype. Furthermore, 73.21% of QTL discovered in the mixed model were determined to cause a crossover interaction effect which cause genotype rank changes between environments. Overall, the QTL discovered via explicitly mapping GxE interactions also explained more yield variance that those QTL associated with differences in traditional stability estimates making their theoretical impact on selection greater. A lack of intersecting results between mapping approaches highlights the importance of examining stability in multiple contexts when attempting to manipulate GxE interactions in soybean.

Keywords: association study; genotype by environment (GxE) interaction; mixed model; soybean; yield stability.