New Bioactive Sesquiterpeniods From the Plant Endophytic Fungus Pestalotiopsis theae

Gaoran Liu; Ruiyun Huo; Yanan Zhai; Ling Liu

doi:10.3389/fmicb.2021.641504

New Bioactive Sesquiterpeniods From the Plant Endophytic Fungus Pestalotiopsis theae

Front Microbiol. 2021 Mar 31:12:641504. doi: 10.3389/fmicb.2021.641504. eCollection 2021.

Authors

Gaoran Liu^{1

2}, Ruiyun Huo^{1

2}, Yanan Zhai¹, Ling Liu^{1

2}

Affiliations

¹ State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
² College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.

Abstract

Three new secondary metabolites pestalothenins A-C (1-3), including two new humulane-derived sesquiterpeniods (1 and 2) and one new caryophyllene-derived sesquiterpeniod (3), together with five known compounds (4-8) were isolated from the crude extract of the plant endophytic fungus Pestalotiopsis theae (N635). Their structures were elucidated by the extensive analyses of HRESIMS and NMR spectroscopic data. The absolute configurations of 1-3 were determined by comparison of experimental and calculated electronic circular dichroism (ECD) spectra. The cytotoxic effects of these compounds were evaluated in vitro. Compound 6 showed moderate cytotoxicity against T24 and MCF7 cell lines. In addition, compounds 1-8 were also evaluated for antibacterial activity.

Keywords: Pestalotiopsis theae; bioactivity; electronic circular dichroism; endophytic fungi; secondary metabolites; sesquiterpenoids.