The mechanism of antigen-presentation of avian bone marrowed dendritic cells suppressed by infectious bronchitis virus

Jinjiao Zuo; Yanan Cao; Zhisheng Wang; Abid Ullah Shah; Wenlei Wang; Chen Dai; Mingjia Chen; Jian Lin; Qian Yang

doi:10.1016/j.ygeno.2021.04.027

The mechanism of antigen-presentation of avian bone marrowed dendritic cells suppressed by infectious bronchitis virus

Genomics. 2021 Jul;113(4):1719-1732. doi: 10.1016/j.ygeno.2021.04.027. Epub 2021 Apr 16.

Authors

Jinjiao Zuo¹, Yanan Cao², Zhisheng Wang³, Abid Ullah Shah², Wenlei Wang¹, Chen Dai¹, Mingjia Chen¹, Jian Lin⁴, Qian Yang²

Affiliations

¹ College of Life Sciences, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu 210095, PR China.
² College of Veterinary Medicine, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu 210095, PR China.
³ Institute of Veterinary Immunology and Engineering, National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China.
⁴ College of Life Sciences, Nanjing Agricultural University, Wei gang 1, Nanjing, Jiangsu 210095, PR China. Electronic address: linjian@njau.edu.cn.

PMID: 33865956
DOI: 10.1016/j.ygeno.2021.04.027

Abstract

Dendritic cells are first guard to defend avian infectious bronchitis virus (IBV) infection and invasion. While IBV always suppress dendritic cells and escape the degradation and presentation, which might help viruses to transfer and migrant. Initially, we compared two IBV's function in activating avian bone marrow dendritic cells (BMDCs) and found that both IBV (QX and M41) did not significantly increase surface marker of avian BMDCs. Moreover, a significant decrease of m⁶A modification level in mRNA, but an increased in the ut RNA were observed in avian BMDCs upon the prevalent IBV (QX) infection. Further study found that both non-structural protein 7 (NSP7) and NSP16 inhibited the maturation and cytokines secretion of BMDCs, as well as their antigen-presentation ability. Lastly, we found that gga-miR21, induced by both NSP7 and NSP16, inhibited the antigen presentation of avian BMDCs. Taken together, our results illustrated how IBV inhibited the antigen-presentation of avian DCs.

Keywords: Avian infectious bronchitis virus; Dendritic cells; m(6)A modification; microRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigen Presentation
Chickens / genetics
Dendritic Cells
Infectious bronchitis virus* / genetics
RNA, Messenger / genetics

Substances

RNA, Messenger