Reversed phase versus hydrophilic interaction liquid chromatography as first dimension of comprehensive two-dimensional liquid chromatography systems for the elucidation of the polyphenolic content of food and natural products

Francesco Cacciola; Katia Arena; Filippo Mandolfino; Danilo Donnarumma; Paola Dugo; Luigi Mondello

doi:10.1016/j.chroma.2021.462129

Reversed phase versus hydrophilic interaction liquid chromatography as first dimension of comprehensive two-dimensional liquid chromatography systems for the elucidation of the polyphenolic content of food and natural products

J Chromatogr A. 2021 May 24:1645:462129. doi: 10.1016/j.chroma.2021.462129. Epub 2021 Mar 31.

Authors

Francesco Cacciola¹, Katia Arena², Filippo Mandolfino², Danilo Donnarumma³, Paola Dugo⁴, Luigi Mondello⁵

Affiliations

¹ Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy. Electronic address: cacciolaf@unime.it.
² Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy.
³ Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy.
⁴ Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy; Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy.
⁵ Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy; Chromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy; Department of Sciences and Technologies for Human and Environment, University Campus Bio-Medico of Rome, 00128 Rome, Italy; BeSep s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy.

PMID: 33864987
DOI: 10.1016/j.chroma.2021.462129

Abstract

Comprehensive two-dimensional liquid chromatography is a well-established method for the unraveling of very complex real-world samples. With regard to food and natural products such a technique turned out to be a very promising approach due to its high resolving power and improved identification capability, especially in combination with mass spectrometry. In this context, polyphenols comprise a particular complex class of bioactive compounds, due to their nature and content in commonly consumed foodstuffs, making their analysis challenging. The present contribution shows an overview of the two commonly employed approaches used for polyphenol analysis, viz. RP-LC × RP-LC and HILIC × RP-LC. Furthermore, the latest implementations as well as limitations and future perspectives are critically reported.

Keywords: Comprehensive two-dimensional liquid chromatography; Food analysis; HILIC × RP-LC; Natural products; Polyphenols; RP-LC × RP-LC.

MeSH terms

Biological Products / chemistry*
Chromatography, Liquid / methods*
Food Analysis / methods*
Hydrophobic and Hydrophilic Interactions
Mass Spectrometry
Polyphenols / analysis*

Substances

Biological Products
Polyphenols