In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination microscope

Xiaofei Qin; Chong Chen; Linbo Wang; Xiaohu Chen; Yong Liang; Xin Jin; Weijun Pan; Zhiying Liu; Hui Li; Guang Yang

doi:10.1016/j.bbrc.2021.03.160

In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination microscope

Biochem Biophys Res Commun. 2021 Jun 11:557:8-13. doi: 10.1016/j.bbrc.2021.03.160. Epub 2021 Apr 12.

Authors

Xiaofei Qin¹, Chong Chen², Linbo Wang², Xiaohu Chen², Yong Liang², Xin Jin², Weijun Pan³, Zhiying Liu¹, Hui Li⁴, Guang Yang⁵

Affiliations

¹ Changchun University of Science and Technology, Changchun, Jilin, 130022, China.
² Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, 215163, China.
³ Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences, Shanghai, 200031, China.
⁴ Changchun University of Science and Technology, Changchun, Jilin, 130022, China; Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, 215163, China.
⁵ Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, 215163, China. Electronic address: yangg@sibet.ac.cn.

PMID: 33857842
DOI: 10.1016/j.bbrc.2021.03.160

Abstract

Precise quantification of vascular developments in Zebrafish requires continuous in-vivo 3D imaging. Here we employed a bi-directional light-sheet illumination microscope to characterize the development process of Zebrafish's intersegmental vessels. A Virtual Reality-based method was used to measure the lengths of intersegmental vessels (ISVs). The quantified growth rates of typical ISVs can be plotted, and unusual growth of some specific vessels was also observed.

Keywords: Light-sheet illumination microscope; Maximum intensity projection; Virtual reality; Zebrafish intersegmental vessel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified
Blood Vessels / embryology*
Blood Vessels / growth & development
Embryo, Nonmammalian / blood supply*
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Imaging, Three-Dimensional / methods
Lighting
Microscopy / instrumentation*
Microscopy / methods
Neovascularization, Physiologic
Zebrafish / embryology*
Zebrafish / growth & development

Substances

enhanced green fluorescent protein
Green Fluorescent Proteins