Prostaglandin-related immune suppression in cattle

Y Sajiki; S Konnai; Y Ikenaka; T Okagawa; N Maekawa; C Logullo; I da Silva Vaz Jr; S Murata; K Ohashi

doi:10.1016/j.vetimm.2021.110238

Prostaglandin-related immune suppression in cattle

Vet Immunol Immunopathol. 2021 Jun:236:110238. doi: 10.1016/j.vetimm.2021.110238. Epub 2021 Apr 6.

Authors

Y Sajiki¹, S Konnai², Y Ikenaka³, T Okagawa⁴, N Maekawa⁴, C Logullo⁵, I da Silva Vaz Jr⁶, S Murata⁷, K Ohashi⁷

Affiliations

¹ Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
² Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan. Electronic address: konnai@vetmed.hokudai.ac.jp.
³ Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan; Translational Research Unit, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Hokkaido University, Kita-18 Nishi-9, Kita-ku, Sapporo, 060-0818, Japan; Water Research Group, Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa.
⁴ Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
⁵ Laboratório Integrado de Bioquímica Hatisaburo Masuda and Laboratório Integrado de Morfologia, NUPEM-UFRJ, Macaé, RJ, Brazil.
⁶ Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, 91501-970, RS, Brazil.
⁷ Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.

PMID: 33857743
DOI: 10.1016/j.vetimm.2021.110238

Abstract

Prostaglandins (PGs) are lipid mediators derived from arachidonic acid by several enzymes including cyclooxygenase (COX)-1 and COX-2. We have previously shown that PGE₂ regulates immune responses, such as Th1 cytokine production and T-cell proliferation, in cattle. However, it is still unclear whether other PGs are involved in the regulation of immune responses in cattle. Here, immunosuppressive profiles of PGs (PGA₁, PGB₂, PGD₂, PGE₂, PGF_1α and PGF_2α) were firstly examined using bovine peripheral blood mononuclear cells (PBMCs). In addition to PGE₂, PGA₁ significantly inhibited Th1 cytokine production from PBMCs in cattle. Further analyses focusing on PGA₁ revealed that treatment with PGA₁ in the presence of concanavalin A (con A) downregulated CD69, an activation marker, and IFN-γ expression in both CD4⁺ and CD8⁺ T cells. Sorted CD3⁺ T cells stimulated with con A were cultivated with PGA₁, and IFN-γ and TNF-α concentrations decreased upon PGA₁ treatment. Taken together, these results suggest that the treatment with PGA₁in vitro inhibits T-cell activation, especially Th1 cytokine production, in cattle.

Keywords: Cattle; Immunosuppression; Prostaglandin; Prostaglandin A(1); Th1 response.

MeSH terms

Animals
Cattle
Cell Proliferation
Immunosuppression Therapy*
Immunosuppressive Agents* / pharmacology
Leukocytes, Mononuclear* / drug effects
Lymphocyte Activation* / drug effects
Prostaglandins* / classification
Prostaglandins* / immunology
Prostaglandins* / pharmacology
Th1 Cells / immunology

Substances

Immunosuppressive Agents
prostaglandin A1
Prostaglandins