Lactobacillus plantarum HFY09 alleviates alcohol-induced gastric ulcers in mice via an anti-oxidative mechanism

Ya Wu; Hong Chen; Yujie Zou; Ruokun Yi; Jianfei Mu; Xin Zhao

doi:10.1111/jfbc.13726

Lactobacillus plantarum HFY09 alleviates alcohol-induced gastric ulcers in mice via an anti-oxidative mechanism

J Food Biochem. 2021 May;45(5):e13726. doi: 10.1111/jfbc.13726. Epub 2021 Apr 13.

Authors

Ya Wu^{1

2

3

4}, Hong Chen⁵, Yujie Zou⁶, Ruokun Yi^{1

2

3}, Jianfei Mu^{1

2

3}, Xin Zhao^{1

2

3}

Affiliations

¹ Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, China.
² Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, China.
³ Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, China.
⁴ College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, China.
⁵ The First Department of Orthopaedic Surgery, Chongqing Traditional Chinese Medicine Hospital, Chongqing, China.
⁶ Department of Emergency, Chongqing University Central Hospital, Chongqing, P.R. China.

PMID: 33846998
DOI: 10.1111/jfbc.13726

Abstract

The protective effect of Lactobacillus plantarum HFY09 (LP-HFY09) on alcohol-induced gastric ulcers was investigated. Gastric morphology observation and pathological tissue sections showed that LP-HFY09 effectively relieved gastric tissue injury. The biochemical indicator detection showed that LP-HFY09 increased superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), glutathione (GSH), prostaglandin E2 (PGE2), and somatostatin (SS) levels, and decreased malondialdehyde (MDA) levels. Moreover, LP-HFY09 inhibited the levels of inflammatory cytokines interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), and elevated the level of anti-inflammatory cytokine IL-10. The quantitative polymerase chain reaction (q-PCR) examination revealed that LP-HFY09 enhanced the mRNA expression of nuclear factor E2-related factor 2 (Nrf2) and downstream genes, including copper/zinc superoxide dismutase (SOD1), heme oxygenase-1 (HO-1), gamma-glutamylcysteine synthetase (GSH1), manganese superoxide dismutase (SOD2), catalase (CAT), and GSH-Px. This study indicated that LP-HFY09 alleviated alcohol-induced gastric ulcers by increasing gastric mucosa defense factor, and inhibiting oxidative stress and the inflammatory response. PRACTICAL APPLICATIONS: LP-HFY09 has the potential to be investigated as a treatment for gastric injury induced by alcohol.

Keywords: Lactobacillus plantarum HFY09; gastric injury; oxidative stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Glutathione Peroxidase / metabolism
Lactobacillus plantarum* / metabolism
Mice
Oxidation-Reduction
Oxidative Stress
Stomach Ulcer* / chemically induced
Stomach Ulcer* / drug therapy

Substances

Glutathione Peroxidase