Photobiomodulation Therapy on Myocardial Infarction in Rats: Transcriptional and Posttranscriptional Implications to Cardiac Remodeling

Regiane Dos Santos Feliciano; Allan Luís Barboza Atum; Érico Gustavo da Silva Ruiz; Andrey Jorge Serra; Ednei Luiz Antônio; Martha Trindade Manchini; Jairo Montemor Augusto Silva; Paulo José Ferreira Tucci; Lubov Nathanson; Mariana Morris; Maria Cristina Chavantes; José Antônio Silva Júnior

doi:10.1002/lsm.23407

Photobiomodulation Therapy on Myocardial Infarction in Rats: Transcriptional and Posttranscriptional Implications to Cardiac Remodeling

Lasers Surg Med. 2021 Nov;53(9):1247-1257. doi: 10.1002/lsm.23407. Epub 2021 Apr 13.

Affiliations

¹ Universidade Nove de Julho, Rua Vergueiro 249, Liberdade, São Paulo, SP, 01504-001, Brazil.
² Universidade Federal de São Paulo, Rua Pedro de Toledo 709, Vila Clementino, São Paulo, SP, 04039-001, Brazil.
³ Nova Southeastern University, 3301 College Avenue, Fort Lauderdale, Florida, 33314.

PMID: 33846991
DOI: 10.1002/lsm.23407

Abstract

Background and objectives: Induction of myocardial infarction (MI) in rats by occlusion of the left anterior descending coronary artery is an experimental model used in research to elucidate functional, structural, and molecular modifications associated with ischemic heart disease. Photobiomodulation therapy (PBMT) has become a therapeutic alternative by modulating various biological processes eliciting several effects, including anti-inflammatory and pro-proliferative actions. The main objective of this work was to evaluate the effect of PBMT in the modulation of transcriptional and post-transcriptional changes that occurred in myocardium signal transduction pathways after MI.

Study design/materials and methods: Continuous wave (CW) non-thermal laser parameters were: 660 nm wavelength, power 15 mW, with a total energy of 0.9 J, fluence of 1.15 J/cm² , spot size of 0.785 cm² , and time of 60 seconds. Using in silico analysis, we selected and then, quantified the expression of messenger RNA (mRNA) of 47 genes of 9 signaling pathways associated with MI (angiogenesis, cell survival, hypertrophy, oxidative stress, apoptosis, extracellular matrix, calcium kinetics, cell metabolism, and inflammation). Messenger RNA expression quantification was performed in myocardial samples by polymerase chain reaction real-time array using TaqMan customized plates.

Results: Our results evidenced that MI modified mRNA expression of several well-known biomarkers related to detrimental cardiac activity in almost all signaling pathways analyzed. However, PBMT reverted most of these transcriptional changes. More expressively, PBMT provoked a robust decrease in mRNA expression of molecules that participate in post-MI inflammation and ECM composition, such as IL-6, TNF receptor, TGFb1, and collagen I and III. Global microRNA (miRNA) expression analysis revealed that PBMT decreased miR-221, miR-34c, and miR-93 expressions post-MI, which are related to deleterious effects in cardiac remodeling.

Conclusion: Thus, the identification of transcriptional and post-transcriptional changes induced by PBMT may be used to interfere in the molecular dynamics of cardiac remodeling post-MI.

Keywords: cardiac remodeling; gene expression; myocardial infarction; photobiomodulation therapy; signal transduction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis
Disease Models, Animal
Low-Level Light Therapy*
MicroRNAs*
Myocardial Infarction* / genetics
Myocardial Infarction* / therapy
Myocardium
Rats
Ventricular Remodeling

Substances

MicroRNAs
Mirn93 microRNA, rat