Characterization of d-xylose reductase, XyrB, from Aspergillus niger

Agata Terebieniec; Tania Chroumpi; Adiphol Dilokpimol; Maria Victoria Aguilar-Pontes; Miia R Mäkelä; Ronald P de Vries

doi:10.1016/j.btre.2021.e00610

Characterization of d-xylose reductase, XyrB, from Aspergillus niger

Biotechnol Rep (Amst). 2021 Mar 15:30:e00610. doi: 10.1016/j.btre.2021.e00610. eCollection 2021 Jun.

Authors

Agata Terebieniec^{1

2}, Tania Chroumpi¹, Adiphol Dilokpimol¹, Maria Victoria Aguilar-Pontes¹, Miia R Mäkelä³, Ronald P de Vries¹

Affiliations

¹ Fungal Physiology, Westerdijk Fungal Biodiversity Institute and Fungal Molecular Physiology, Utrecht University, Utrecht, the Netherlands.
² Department of Molecular Biotechnology and Microbiology, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland.
³ Department of Microbiology, University of Helsinki, Helsinki, Finland.

Abstract

d-xylose reductase is a member of the aldo-keto reductase family, and is involved in d-xylose and l-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second d-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert d-xylose and l-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).

Keywords: AKR, aldo-keto reductase; Aspergillus niger; CtXR, Candida tenuis xylose reductase; GCY1/YPR1, yeast glycerol dehydrogenases; GldB, filamentous fungal glycerol dehydrogenase; IPTG, isopropyl β-D-1-thiogalactopyranoside; LB, Luria Bertani; LadA, l-arabitol dehydrogenase; LarA, l-arabinose reductase; LxrA, LxrB, l-xylulose reductase; NAD, nicotinamide adenine dinucleotide; NADP, nicotinamide adenine dinucleotide phosphate; NADPH, reduced; PBS, phosphate buffered saline; PCP, Pentose Catabolic Pathway; PCR, polymerase chain reaction; PPP, Pentose Phosphate Pathway; PRD1, pentose reductase; Pentose Catabolic Pathway; SdhA, sorbitol dehydrogenase; XdhA, xylitol dehydrogenase; XkiA, d-xylulose kinase; XyrA, XyrB, d-xylose reductase; d-xylose reductase.