Microalgae immobilized in hydrogels offer advantages over those cultured in suspension culture in terms of carbon fixation and oxygen emission. However, alginate as a commonly used hydrogel for microalgal immobilization encounters problems with mechanical strength and stability. To address this limitation, silk fibroin (silk) hydrogels prepared by ultrasonication were utilized to host microalgae when mixed with the presonicated protein solution prior to its gelation. The gelation time, stability, and light transmission of these silk gels were evaluated, and a silk concentration of 4% w/v and a gel thickness of 1 mm provided mechanical strength and stability during algal culture in comparison to alginate hydrogels. Furthermore, silk hydrogels with algal cell densities of 7.6 × 105 and 7.8 × 107 cells/mL had better stability than those with a lower cell density (3.2 × 103 cells/mL), likely due to cell confinement and impact on proliferation. The silk hydrogels with microalgae at a high density generated 6.13 mg/L of oxygen continuously for 7 days. An oxygen-generating device was fabricated by coating the surface of a dialysis tube with a thin layer of the microalgae-embedded silk hydrogel, where the microalgal cells were nourished with culture medium prefilled in the dialysis tube. When suspended in a sealed flask filled with CO2 gas, the system continuously produced oxygen (151 mL) for at least 60 days, with an oxygen production efficiency 6 times that of microalgal suspension culture controls. This microalgae embedding and cultivation technique could have potential utility in air purification, tissue repair, and other applications due to the efficient and sustained generation of oxygen.
Keywords: air purification; hydrogel; microalgae; oxygen production; silk.