Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs

Yan Lu; Yi Xiao; Ming-Zhu Yin; Xing-Chen Zhou; Li-Sha Wu; Wang-Qing Chen; Yan Luo; Ye-Hong Kuang; Wu Zhu

doi:10.3389/fmed.2020.560579

Polyethylene Glycol Ointment Alleviates Psoriasis-Like Inflammation Through Down-Regulating the Function of Th17 Cells and MDSCs

Front Med (Lausanne). 2021 Mar 22:7:560579. doi: 10.3389/fmed.2020.560579. eCollection 2020.

Authors

Yan Lu^{1

2}, Yi Xiao^{1

2}, Ming-Zhu Yin^{1

2}, Xing-Chen Zhou³, Li-Sha Wu^{2

4}, Wang-Qing Chen¹, Yan Luo^{1

2}, Ye-Hong Kuang^{1

2}, Wu Zhu^{1

2}

Affiliations

¹ Department of Dermatology, Xiangya Hospital, Central South University, Changsha, China.
² Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, China.
³ Pharmacy, Xiangya Hospital, Central South University, Changsha, China.
⁴ Institute of Medical Science, Xiangya Hospital, Central South University, Changsha, China.

Abstract

Objective: To explore the possible mechanism of improving the imiquimod (IMQ)-induced psoriasis-like inflammation by using polyethylene glycol (PEG) ointment. Methods: We evaluated the appearance of psoriasis lesions by Psoriasis Area and Severity Index (PASI), observed the epidermal proliferation by histopathological staining and immunohistochemical staining, and explored the key molecules and signaling pathways of improving psoriasis-like inflammation treated with PEG ointment by RNA sequencing. Finally, we verified the expression of inflammatory cells and inflammatory factors by flow cytometry, immunohistochemical staining, and Q-PCR. Results: PEG ointment could improve the appearance of psoriasis lesions and the epidermis thickness of psoriasis mouse, inhibit the proliferation of keratinocytes, and down-regulate the relative mRNA levels of IL-23, IL-22, IL-6, IL-17C, IL-17F, S100A7, S100A8, S100A9, CXCL1, CXCL2, and IL-1β in the skin lesions of psoriasis mouse by down-regulating the numbers of myeloid-derived suppressor cells (MDSCs) and T helper 17 (Th17) cells. Conclusion: PEG ointment could improve the IMQ-induced psoriasis-like inflammation by down-regulating the functions of Th17 cells and MDSCs.

Keywords: IMQ; MDSCs; PEG; Th17; psoriasis.