Curcumin Ameliorates Palmitic Acid-Induced Saos-2 Cell Apoptosis Via Inhibiting Oxidative Stress and Autophagy

Baicheng Ma; Gaopeng Guan; Qizhuang Lv; Lei Yang

doi:10.1155/2021/5563660

Curcumin Ameliorates Palmitic Acid-Induced Saos-2 Cell Apoptosis Via Inhibiting Oxidative Stress and Autophagy

Evid Based Complement Alternat Med. 2021 Mar 26:2021:5563660. doi: 10.1155/2021/5563660. eCollection 2021.

Authors

Baicheng Ma^{1

2}, Gaopeng Guan^{1

3}, Qizhuang Lv⁴, Lei Yang¹

Affiliations

¹ Jiangxi Provincial Key Lab of System Biomedicine, Jiujiang University, Jiujiang 332000, Jiangxi, China.
² School of Medicine, Jiujiang University, Jiujiang 332000, Jiangxi, China.
³ Affiliated Hospital of Jiujiang University, Jiujiang University, Jiujiang 332000, Jiangxi, China.
⁴ College of Biology & Pharmacy, Yulin Normal University, Yulin 537000, Guangxi, China.

Abstract

Objectives: We aimed to determine the effects of curcumin on palmitic acid- (PA-) induced human osteoblast-like Saos-2 cell apoptosis and to explore the potential molecular mechanisms in vitro level.

Methods: Saos-2 cell were cultured with PA with or without curcumin, N-acetylcysteine (NAC, anti-oxidant), 3-methyladenine (3-MA, autophagy inhibitor) AY-22989 (autophagy agonist) or H₂O₂. Then, the effects of PA alone or combined with curcumin on viability, apoptosis, oxidative stress, and autophagy in were detected by CCK-8, flow cytometry assay and western blot.

Results: We found that autophagy was induced, oxidative stress was activated, and apoptosis was promoted in PA-induced Saos-2 cells. Curcumin inhibited PA-induced oxidative stress, autophagy, and apoptosis in Saos-2 cells. NAC successfully attenuated oxidative stress and apoptosis, and 3-MA attenuated oxidative stress and apoptosis in palmitate-induced Saos-2 cells. Interestingly, NAC inhibited PA-induced autophagy, but 3-MA had no obvious effects on oxidative stress in PA-treated Saos-2 cells. In addition, curcumin inhibited H₂O₂ (oxidative stress agonist)-induced oxidative stress, autophagy, and apoptosis, but curcumin had no obvious effect on AY-22989 (autophagy agonist)-induced autophagy and apoptosis.

Conclusion: The present study demonstrated that oxidative stress is an inducer of autophagy and that curcumin can attenuate excess autophagy and cell apoptosis by inhibiting oxidative stress in PA-induced Saos-2 cells.