On the molecular identity of Paratylenchus nanus (Nematoda: Tylenchida)

Sergei A Subbotin; Guiping Yan; Mihail Kantor; Zafar Handoo

doi:10.21307/jofnem-2020-127

On the molecular identity of Paratylenchus nanus (Nematoda: Tylenchida)

J Nematol. 2021 Jan 11:52:e2020-127. doi: 10.21307/jofnem-2020-127. eCollection 2020.

Authors

Sergei A Subbotin^{1

2}, Guiping Yan³, Mihail Kantor⁴, Zafar Handoo⁴

Affiliations

¹ Plant Pest Diagnostic Center, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, CA 95832.
² Center of Parasitology of A.N. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Leninskii Prospect 33, Moscow 117071, Russia.
³ Department of Plant Pathology, North Dakota State University, Fargo, ND 58108.
⁴ Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Building 010 A, BARC-West, 10300 Baltimore Avenue, Beltsville, MD 20705.

Abstract

In this study, molecular characterization of Paratylenchus nanus collected from the type locality in Four Mile Run, Fall Church, Virginia using COI, D2-D3 of 28 S rRNA and ITS rRNA gene sequences was provided. We molecularly also characterized, Paratylenchus specimens collected from grasses in Devils Lake, Ramsey County, North Dakota indicated as the type locality in the original description of P. nanus by Cobb (1923). These nematodes were identified as representatives of the species P. projectus. Populations of P. nanus belonging to the molecular types A and B, and previously designated by Van den Berg et al. (2014) should be now identified as P. nanus and P. projectus, respectively.

Keywords: COI; D2-D3 of 28S rRNA; ITS rRNA; Molecular phylogeny; P. nanus type A; P. nanus type B; Paratylenchus projectus.