Phylogenetic characterization of avian pathogenic Escherichia coli strains longitudinally isolated from broiler breeder flocks vaccinated with autogenous vaccine

Abstract

Escherichia coli is the most common bacterial cause of infections in poultry farms. It is known for its genetic heterogenicity that complicates the protection of poultry health through immunoprophylaxis. In farms with continuous problems with colibacillosis, autogenous E. coli vaccine was implemented to the vaccination program instead of commercial vaccines. In this study, we investigated the effect of the autogenous vaccine on E. coli phylogroup diversity on 2 broiler breeder farms with 4 and 5 flocks, respectively. The first flocks on both farms were vaccinated with commercial vaccines, while application of autogenous vaccine was introduced in the second flock on both farms. In total, 113 strains were selected based on the target organs and age of chickens. Targeted organs were the peritoneum, liver, oviduct, and bone marrow, and analyzed strains were isolated from chickens older than 21 wk of age when problems with colibacillosis start emerging. The strains were phylotyped by PCR and allocated to phylogroups A, B1, B2, C, D, E, F or clades I-V. The results showed that autogenous vaccine could significantly affect the phylogroup shift of the strains. On farm A, application of the autogenous vaccine induced significantly lower prevalence (P = 0.01) of the phylogroups represented in the vaccine among the strains later isolated from the vaccinated flock, while on farm B, the results showed a decrease in the phylogenetic diversity with a dominant prevalence of group B2 despite the vaccine application. The results indicate that implementation of the autogenous vaccine can repress the majority of the strains, but also be unable to eliminate the presence of certain phylogroups, and thus lead to strain shift. Further detailed analyses of multilocus sequence typing and virulence genes will elucidate the pathogenic potential and selection of certain strains, with emphasis on B2 phylogroup.

Keywords: Escherichia coli; PCR; autogenous vaccine; phylotyping; poultry.