Metagenomics reveals functional profiling of microbial communities in OCP contaminated sites with rapeseed oil and tartaric acid biostimulation

Zhongyun Zhang; Jinzhong Wan; Li Liu; Mao Ye; Xin Jiang

doi:10.1016/j.jenvman.2021.112515

Metagenomics reveals functional profiling of microbial communities in OCP contaminated sites with rapeseed oil and tartaric acid biostimulation

J Environ Manage. 2021 Jul 1:289:112515. doi: 10.1016/j.jenvman.2021.112515. Epub 2021 Apr 2.

Authors

Zhongyun Zhang¹, Jinzhong Wan², Li Liu³, Mao Ye⁴, Xin Jiang⁵

Affiliations

¹ Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
² Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing, 210008, China.
³ Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing, 210008, China; School of Earth Science and Engineering, Hohai University, Nanjing, 210008, China.
⁴ Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. Electronic address: yemao@issas.ac.cn.
⁵ Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.

PMID: 33819653
DOI: 10.1016/j.jenvman.2021.112515

Abstract

Organochlorine pesticides (OCPs) contaminated sites pose great threats to both human health and environmental safety. Targeted bioremediation in these regions largely depends on microbial diversity and activity. This study applied metagenomics to characterize the microbial communities and functional groups composition features during independent or simultaneous rapeseed oil and tartaric acid applications, as well as the degradation kinetics of OCPs. Results showed that: the degradation rates of α-chlordane, β-chlordane and mirex were better when (0.50% w/w) rapeseed oil and (0.05 mol L^-1) tartaric acid were applied simultaneously than singular use, yielding removal rates of 56.4%, 53.9%, and 49.4%, respectively. Meanwhile, bio-stimulation facilitated microbial enzyme (catalase/superoxide dismutase/peroxidase) activity in soils significantly, promoting the growth of dominant bacterial communities. Classification at phylum level showed that the relative abundance of Proteobacteria was significantly increased (p < 0.05). Network analysis showed that bio-stimulation substantially increased the dominant bacterial community's proportion, especially Proteobacteria. The functional gene results illustrated that bio-stimulation facilitated total relative abundance of degradation genes, phosphorus, carbon, nitrogen, sulfur metabolic genes, and iron transporting genes (p < 0.05). In metabolic pathways, functional genes related to methanogenesis and ammonia generation were markedly upregulated, indicating that bio-stimulation promoted the transformation of metabolic genes, such as carbon and nitrogen. This research is conducive to exploring the microbiological response mechanisms of bio-stimulation in indigenous flora, which may provide technical support for assessing the microbial ecological remediation outcomes of bio-stimulation in OCP contaminated sites.

Keywords: Bio-stimulation; Metagenomics; Microbial communities; Organochlorine pesticide; Rapeseed oil; Tartaric acid.

MeSH terms

Biodegradation, Environmental
Humans
Metagenomics
Microbiota*
Rapeseed Oil
Soil Microbiology
Soil Pollutants* / analysis
Tartrates

Substances

Rapeseed Oil
Soil Pollutants
Tartrates
tartaric acid