CD47-mediated DTIC-loaded chitosan oligosaccharide-grafted nGO for synergistic chemo-photothermal therapy against malignant melanoma

Xiaozhen Zhan; Wanqing Teng; Kai Sun; Jiexiang He; Jie Yang; Jinhuan Tian; Xun Huang; Lin Zhou; Changren Zhou

doi:10.1016/j.msec.2021.112014

CD47-mediated DTIC-loaded chitosan oligosaccharide-grafted nGO for synergistic chemo-photothermal therapy against malignant melanoma

Mater Sci Eng C Mater Biol Appl. 2021 Apr:123:112014. doi: 10.1016/j.msec.2021.112014. Epub 2021 Mar 5.

Authors

Xiaozhen Zhan¹, Wanqing Teng², Kai Sun³, Jiexiang He³, Jie Yang⁴, Jinhuan Tian⁵, Xun Huang⁶, Lin Zhou⁷, Changren Zhou⁸

Affiliations

¹ The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, PR China. Electronic address: zxz2019@jnu.edu.cn.
² Collaborative Innovation Center of Tumor Marker Detection Technology, Equipment and Diagnosis-Therapy Integration in Universities of Shandong, Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, Shandong 276005, PR China.
³ College of Chemistry and Material Science, Jinan University, Guangzhou, Guangdong 510632, PR China.
⁴ The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, PR China.
⁵ College of Chemistry and Material Science, Jinan University, Guangzhou, Guangdong 510632, PR China. Electronic address: jhtian@jnu.edu.cn.
⁶ Collaborative Innovation Center of Tumor Marker Detection Technology, Equipment and Diagnosis-Therapy Integration in Universities of Shandong, Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi, Shandong 276005, PR China. Electronic address: xhuang2019@126.com.
⁷ The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510632, PR China. Electronic address: zhoulin0801@jnu.edu.cn.
⁸ College of Chemistry and Material Science, Jinan University, Guangzhou, Guangdong 510632, PR China. Electronic address: tcrz9@jnu.edu.cn.

PMID: 33812633
DOI: 10.1016/j.msec.2021.112014

Abstract

Nano-graphene oxide (nGO), an effective drug nanocarrier, is used for simultaneous photothermal therapy (PTT) and near-infrared fluorescence imaging. Dacarbazine (DTIC) is used in the treatment of melanoma with limited clinical efficacy. PTT shows promise in the treatment of skin cancer. Herein, chitosan oligosaccharide (COS)-grafted nGO was further modified with CD47 antibody, and loaded DTIC was prepared using a versatile nanoplatform (nGO-COS-CD47/DTIC) for the treatment of melanoma as a synergistic targeted chemo-photothermal therapy. The in vitro results demonstrated that nGO-COS-CD47/DTIC nanocarriers have excellent biocompatibility, photothermal conversion efficiency, high targeting efficiency, fast drug release under NIR irradiation, and tumor cell killing efficiency. Notably, nGO-COS-CD47/DTIC plus NIR irradiation significantly promoted early cell apoptosis through the mitochondrial apoptosis pathway and exhibited a significant joint function of antitumor efficacy. The demonstrated nGO-COS-CD47/DTIC can provide a highly efficient malignant melanoma therapy using this multifunctional intelligent nanoplatform.

Keywords: CD47; Chemo-photothermal therapy; Chitosan oligosaccharide; Dacarbazine; Melanoma; Nano-graphene oxide.

MeSH terms

CD47 Antigen
Cell Line, Tumor
Chitosan*
Dacarbazine
Doxorubicin
Humans
Melanoma* / drug therapy
Nanoparticles*
Oligosaccharides / pharmacology
Phototherapy
Photothermal Therapy
Skin Neoplasms*

Substances

CD47 Antigen
CD47 protein, human
Oligosaccharides
Dacarbazine
Doxorubicin
Chitosan